肠道屏障功能对机体健康至关重要，生理状态下，肠道发挥屏障功能可有效防止机体内环境受到有害物质的侵害，保障机体内环境稳定。当各种因素使机体内环境受到侵害，可导致肠道屏障功能受损，肠道通透性增加，从而出现肠道内菌群移位、内毒素吸收等一系列病理改变。本文对相关文献进行检索、整理、归纳发现肠道屏障功能的检测与评价方法较多，通过测定血液、尿液、粪便或肠道组织等标本中的某些指标，从体内、体外2个方面对肠道屏障功能进行评价，总结了肠道屏障功能的评价方法，并分析了每种评价方法的优缺点，以期为后续此领域的研究提供参考。

Intestinal barrier function is very important for overall health. Under physiological conditions，intestinal barrier can effectively prevent the internal environment from being affected by harmful substances and ensure the stability of the internal environment. When the internal environment of the body is damaged by various factors，the intestinal barrier function can be impaired，leading to increased intestinal permeability and thus a series of pathological changes such as intestinal flora shift and endotoxin absorption. By searching，sorting，and summarizing relevant literature，the authors found that there are many methods for detection and evaluation of intestinal barrier function. Indicators measured using blood，urine，stool，or intestinal tissue samples have been used to evaluate intestinal barrier function both in vivo and in vitro. This paper summarizes the methods of intestinal barrier function evaluation，and analyzes the advantages and disadvantages of each evaluation method，in order to provide a reference for the subsequent research in this field.