猴痘病毒实验室检测技术研究进展

周建, 任雪梅, 王馨, 李卓

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重庆医科大学学报 ›› 2024, Vol. 49 ›› Issue (11) : 1367-1371. DOI: 10.13406/j.cnki.cyxb.003613
综述

猴痘病毒实验室检测技术研究进展

作者信息 +

Research advances in laboratory detection techniques for monkeypox virus

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摘要

目前猴痘病毒疫情传播已打破地域流行的规律,呈现世界范围内流行的特点。因此猴痘疫情的防控成为全世界关注的焦点问题,而引入有效的实验室检测方法则是疫情防控的关键。目前针对猴痘病毒的检测已有一套完备的方法,包括病毒分离培养,抗原抗体检测以及核酸检测等,其中用于核酸检测的技术手段又包括聚合酶链式反应、等温扩增以及新兴的CRISPR/Cas技术等。本文主要针对当前猴痘病毒实验室检测技术的原理、特点和缺陷进行综述分析,期望为猴痘病毒疫情防控提供参考依据。

Abstract

At present,the spread of monkeypox virus epidemic breaks the law of regional prevalence and shows the characteristics of worldwide prevalence. Therefore,precontrol of monkeypox epidemic has become a key issue around the world,and an effective laboratory virus detection method is the key to epidemic prevention and control. There is currently a set of complete procedures for the detection of monkeypox virus,involving virus isolation and culture,detection of antigens and antibodies,and nucleic acid detection with the methods such as polymerase chain reaction,isothermal amplification, and the emerging CRISPR/Cas nucleic acid detection technique. This article reviews the principles,characteristics and defects of the laboratory detection of monkeypox virus,so as to provide a reference for the prevention and control of monkeypox virus epidemic.

关键词

猴痘病毒 / 实验室检测 / CRISPR/Cas

Key words

monkeypox virus / laboratory detection / CRISPR/Cas

中图分类号

R446

引用本文

导出引用
周建 , 任雪梅 , 王馨 , . 猴痘病毒实验室检测技术研究进展. 重庆医科大学学报. 2024, 49(11): 1367-1371 https://doi.org/10.13406/j.cnki.cyxb.003613
Zhou Jian, Ren Xuemei, Wang Xin, et al. Research advances in laboratory detection techniques for monkeypox virus[J]. Journal of Chongqing Medical University. 2024, 49(11): 1367-1371 https://doi.org/10.13406/j.cnki.cyxb.003613

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基金

中国博士后科学基金资助项目(2020M670074ZX)
西安市创新能力强基计划-医学研究资助项目(22YXYJ0126)

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