目的 探究氟苯尼考（florfenicol，FLO）暴露对蜕膜化的影响及线粒体在其中发挥的作用。 方法 构建FLO暴露的原代小鼠蜕膜基质细胞（mouse decidual stromal cells，mDSCs）模型，经FLO（1.56、6.25、25.00 μg/mL）暴露后，采用鬼笔环肽染色法观察细胞形态变化，采用Western blot检测蜕膜化标志分子HOXA10表达以分析FLO对蜕膜化的影响；采用流式细胞术检测细胞凋亡，采用Western blot检测细胞增殖标志分子PCNA表达以分析FLO对蜕膜化过程中细胞增殖、凋亡的影响；采用荧光探针Mito-tracker Red观察线粒体形态，采用免疫荧光检测线粒体外膜标志蛋白TOMM20表达并检测ATP水平以分析FLO对蜕膜基质细胞线粒体功能的影响。 结果 25.00 μg/mL FLO暴露导致细胞形态异常，蜕膜化标志分子HOXA10表达降低（P=0.002），细胞凋亡水平增加，细胞增殖能力降低；进一步发现FLO暴露后线粒体外膜标志蛋白TOMM20表达降低（P=0.010）、线粒体形态异常和ATP水平降低（P=0.003）。 结论 25.00 μg/mLFLO暴露可能与线粒体功能打破mDSCs的细胞增殖、凋亡平衡，损伤蜕膜化进程有关。

Objective To investigate the impact of florfenicol（FLO） exposure on decidualization and the role of mitochondria in this process. Methods Primary mouse decidual stromal cells（mDSCs）were used to establish a model of FLO exposure. After exposure to FLO（1.56，6.25，and 25.00 μg/mL），Phalloidin staining was used to observe cell morphology；Western blot was used to measure the expression of HOXA10，a maker molecule for decidualization； and investigate the influence of FLO on cell proliferation and apoptosis during decidualization；the fluorescent probe Mito-tracker Red was used to observe mitochondrial morphology； immunofluorescence assay was used to measure the expression of TOMM20，a marker protein for outer mitochondrial membrane，and the level of ATP to observe the influence of FLO on mitochondrial function in mDSCs. Results Exposure to 25.00 μg/mL FLO led to abnormal cell morphology，reduced the expression of the marker molecule for decidualization HOXA10（P=0.002），promoted apoptosis，and inhibited cell proliferation. Further analysis showed a reduction in the expression of marker protein for outer mitochondrial membrane TOMM20（P=0.010），abnormal mitochondrial morphology，and a reduction in the level of ATP（P=0.003）. Conclusion Exposure to 25.00 μg/mL FLO may disrupt the balance between the proliferation and apoptosis of mDSCs and damage the decidualization process，which is associated with mitochondrial function.