Objective To explore the effectiveness of "lecture-based learning+case-based learning+problem-based learning" three-track teaching method combined with multimedia courseware in tumor immunology. Methods A total of 132 undergraduates from Capital Medical University were included, and divided into experimental group (85 students) and control group (47 students). Three-track teaching method combined with multimedia courseware was used in the experimental group while lecture-based learning was used in the control group. The test and questionnaire survey were performed to evaluate teaching effectiveness. Results Both the test score and the proportion of excellent students were higher in the experimental group than those in the control group (both P＜0.001). Furthermore, a questionnaire survey performed in the experimental group showed that satisfaction rate of the students on the contents, clinical thinking cultivation, and teaching effectiveness for three-track teaching method combined with multimedia courseware in tumor immunology were 97.5%, 93.8%, and 95.1%, respectively. Conclusion Compared to lecture-based learning, three-track teaching method combined with multimedia courseware has a better teaching effectiveness in tumor immunology, and can improve the teaching quality.