Objective To evaluate the application of standardized training system in the refresher training in Pathology Department. Methods Seventy continuing education students of the Department of Pathology, Renmin Hospital of Wuhan University from January 2016 to August 2023 were selected as the research subjects. Before the teaching reform, 28 continuing education students were selected as the traditional group and received traditional training mode. After the teaching reform, 42 continuing education students were selected as the teaching reform group and received standardized training mode. We conduct theoretical and practical teaching effectiveness evaluation, scientific research evaluation, and teaching satisfaction evaluation through graduation assessment, scientific research paper publication statistics, and questionnaire survey. Results The average score of the graduation assessment for the teaching reform group students was (98.60±1.43) points, while the average score of the traditional group students was (93.18±3.71) points, with a statistically significant difference (P＜0.0001). The number of articles published by students in the teaching reform group is much higher than that of students in the traditional group. A teaching satisfaction survey conducted in the teaching reform group showed that the satisfaction of the students on the management of education, learning resources, tutors and research guidance was 100%. Conclusion The standardized training system in the refresher training can significantly improve the clinical comprehensive level of continuing education students, enhance their scientific research ability, and improve teaching satisfaction, which is worth promoting and applying in clinical teaching.