PDF(1628 KB)
增益染色吸光法检测水中低浓度微纳塑料
雷莹莹, 王昭然, 陈寒玉
PDF(1628 KB)
PDF(1628 KB)
增益染色吸光法检测水中低浓度微纳塑料
Detection of Low Concentration of Micro-nano Plastics in Water by Gain-stained Absorbance Method
按照染料增益吸光性的原理建立新的光谱法测定水中微塑料含量，具有操作简便、快速、灵敏度高和可选择性检测微纳碎片等优点。采用不同粒径、不同种类塑料作为测试材料，结果显示均有良好的增益吸光效果。方法的适用性研究显示，对于粒径大于1 μm的微塑料颗粒和纤维混合体，可在染色、离心后取定量溶液在血球计数板上，采用显微荧光计数法测定其含量；对于粒径小于1 μm的纳塑料，可利用其染色后悬浮相浓度和吸光度呈现的线性关系，采用分光光度法进行测定。将该检测方法应用于某污水处理厂实际水样中，结果显示，实际样品加标回收率在99.00%~107.00%之间，方法检测限可达1.53 mg/L。研究将为环境中微纳塑料的定量检测提供一种便利的方法，具有良好的适用性和广阔的应用前景。
A new spectroscopic method for the determination of microplastics based on the principle of dye-gain absorption has the advantages of simplicity, rapidity, high sensitivity and selective detection of micro-nano fragments. Different particle sizes and types of plastics were used as test materials, and the results showed good gain absorbance. The applicability of the method showed that for the microplastic particles and fiber mixtures with the particles larger than 1 μm, the concentration could be determined by staining, centrifugation, and then taking a quantitative solution on a hemocyte counting plate by microfluorescence counting. For the nanoplastics with the particles lower than 1 μm, the spectrophotometric method can be employed for measurement by utilizing the linear relationship between the concentration of the suspended phase after staining and its absorbance. The method was applied to actual water samples from a wastewater treatment plant, and the results showed that the spiked recoveries of the actual samples ranged from 99.00% to 107.00%, and the detection limit of the method was up to 1.53 mg/L.The present study will provide a convenient method for the quantitative detection of micro-nano plastics in the environment, which has good applicability and wide application prospects.
Microplastics / Detection method / Staining / Disperse red 3B
O657.32
| 1 |
朱令楠,李艳博,郭彩霞.环境微塑料呼吸道暴露及其健康效应研究进展[J].中国环境科学,2024,44(2):1134-1143.
|
| 2 |
王晓雄,刘心悦.空气中微塑料的危害与治理[J].生态经济,2023,39(12):5-8.
|
| 3 |
吴泽隆,胡良,郭若彬,等.农田土壤中的微塑料研究进展[J].农业与技术,2024,44(1):86-90.
|
| 4 |
李道季,蒋春华.海洋中的塑料垃圾和微塑料:从污染到治理[J].世界科学,2023(12):33-35.
|
| 5 |
秦潇,周颖,黄艺.微塑料的人群暴露途径及其健康危害[J].生态毒理学报,2021,16(5):221-227.
|
| 6 |
孙梦瑶,郭家阳,王欣奕,等.微塑料来源、分布及其对植物、动物和人体危害研究进展[J].应用生态学报,2024,35(8):2301-2312.
|
| 7 |
孙香莹,庄银,王玉邦,等.微塑料环境暴露与人体健康效应研究进展[J].环境科学研究,2023,36(5):1020-1031.
|
| 8 |
谢颖,杨波,刘琳豆,等.微塑料的人体内外暴露现状及其检测技术进展[J].环境卫生学杂志,2023,13(10):778-787.
|
| 9 |
|
| 10 |
|
| 11 |
|
| 12 |
李伟.警惕进入人体的微塑料[J].检察风云,2024(9):36-37.
|
| 13 |
孙一喆,詹健.水环境中微塑料去除技术研究进展[J].应用化工,2024,53(2):447-452.
|
| 14 |
王嘉嘉,王佩瑶,王成浩,等.土壤中微塑料的检测及其对土壤生态环境的影响[J].塑料科技,2022,50(10):108-112.
|
| 15 |
王兴,邓玉明,宋利君,等.ATR及热分析法在塑料食品包装质控方面应用的研究[J].农产品加工,2017(11):12-14, 19.
|
| 16 |
章勇.水环境微塑料分析方法研究进展[J].安徽农学通报,2024,30(5):62-65.
|
| 17 |
万青云,黄燕平,秦嘉,等.环境中微塑料检测分析方法研究进展[J].环境监测管理与技术,2024,36(2):1-6.
|
| 18 |
薛晓熠,沈之霞.微塑料的主要来源及其检测方法研究进展[J].塑料包装,2023,33(6):39-45.
|
| 19 |
刘禹慧,韩卓,巢静波,等.热分析质谱技术在微塑料检测领域的应用[J].中国无机分析化学,2024,14(8):1166-1176.
|
| 20 |
卢琦园,马艳,陈思帆,等.环境中微塑料检测方法的研究进展[J].环境化学,2024,43(7):2166-2180.
|
| 21 |
贾春荣,孟淼,高建鑫,等.水体中微塑料的光谱检测研究和应用[J].激光杂志,2025,46(1):9-15.
|
| 22 |
肖铮,贺仪,苑林宏,等.水环境中微塑料的来源分布及其分子光谱检测技术的研究进展[J].中国无机分析化学,2024,14(8):1147-1156.
|
| 23 |
崔胜东,李永增,彭伟金,等.饮用水中的微塑料及其拉曼光谱检测技术研究进展[J].激光生物学报,2024,33(1):14-23.
|
| 24 |
张万万,郑永杰,田景芝.罗丹明B染色法快速测定土壤中微塑料[J].齐齐哈尔大学学报:自然科学版,2022,38(2):77-83.
|
| 25 |
胥文瑞,周李俊君,王超,等.尼罗红染色聚苯乙烯微塑料新方法开发[J].塑料科技,2024,52(4):49-55.
|
| 26 |
朱善长.浅论塑料染色的染料选样与应用[J].染料工业,1988(3):52-56.
|
| 27 |
苗令占,邓肖雅,杨铮,等.微塑料的老化过程、产物及其环境效应研究进展[J].中国环境科学,2023,43(11):6156-6171.
|
| 28 |
张倩芝,陈晓红.基于显微红外光谱法鉴定室内空气沉降物中的微塑料[J].光谱学与光谱分析,2023,43(S1):7-8.
|
| 29 |
牟可磊.亲核取代改性蒽醌染料原液着色性能研究[D].上海:东华大学,2022.
|
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