增益染色吸光法检测水中低浓度微纳塑料

雷莹莹, 王昭然, 陈寒玉

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塑料科技 ›› 2025, Vol. 53 ›› Issue (02) : 131-135. DOI: 10.15925/j.cnki.issn1005-3360.2025.02.024
理化测试

增益染色吸光法检测水中低浓度微纳塑料

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Detection of Low Concentration of Micro-nano Plastics in Water by Gain-stained Absorbance Method

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摘要

按照染料增益吸光性的原理建立新的光谱法测定水中微塑料含量,具有操作简便、快速、灵敏度高和可选择性检测微纳碎片等优点。采用不同粒径、不同种类塑料作为测试材料,结果显示均有良好的增益吸光效果。方法的适用性研究显示,对于粒径大于1 μm的微塑料颗粒和纤维混合体,可在染色、离心后取定量溶液在血球计数板上,采用显微荧光计数法测定其含量;对于粒径小于1 μm的纳塑料,可利用其染色后悬浮相浓度和吸光度呈现的线性关系,采用分光光度法进行测定。将该检测方法应用于某污水处理厂实际水样中,结果显示,实际样品加标回收率在99.00%~107.00%之间,方法检测限可达1.53 mg/L。研究将为环境中微纳塑料的定量检测提供一种便利的方法,具有良好的适用性和广阔的应用前景。

Abstract

A new spectroscopic method for the determination of microplastics based on the principle of dye-gain absorption has the advantages of simplicity, rapidity, high sensitivity and selective detection of micro-nano fragments. Different particle sizes and types of plastics were used as test materials, and the results showed good gain absorbance. The applicability of the method showed that for the microplastic particles and fiber mixtures with the particles larger than 1 μm, the concentration could be determined by staining, centrifugation, and then taking a quantitative solution on a hemocyte counting plate by microfluorescence counting. For the nanoplastics with the particles lower than 1 μm, the spectrophotometric method can be employed for measurement by utilizing the linear relationship between the concentration of the suspended phase after staining and its absorbance. The method was applied to actual water samples from a wastewater treatment plant, and the results showed that the spiked recoveries of the actual samples ranged from 99.00% to 107.00%, and the detection limit of the method was up to 1.53 mg/L.The present study will provide a convenient method for the quantitative detection of micro-nano plastics in the environment, which has good applicability and wide application prospects.

关键词

微塑料 / 检测方法 / 染色 / 分散红3B

Key words

Microplastics / Detection method / Staining / Disperse red 3B

中图分类号

O657.32

引用本文

导出引用
雷莹莹 , 王昭然 , 陈寒玉. 增益染色吸光法检测水中低浓度微纳塑料. 塑料科技. 2025, 53(02): 131-135 https://doi.org/10.15925/j.cnki.issn1005-3360.2025.02.024
LEI Yingying, WANG Zhaoran, CHEN Hanyu. Detection of Low Concentration of Micro-nano Plastics in Water by Gain-stained Absorbance Method[J]. Plastics Science and Technology. 2025, 53(02): 131-135 https://doi.org/10.15925/j.cnki.issn1005-3360.2025.02.024

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基金

河南省科技厅联合基金项目(232103810070)

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