目的 诊断贵州省某生猪养殖场仔猪异常发病死亡原因，为生猪养殖场疾病防控提供参考。 方法 无菌采集2头3~14日龄患病仔猪的心脏、肝脏、脾脏、肺脏、肾脏、淋巴结等组织，采用临床观察、病理剖解、细菌分离培养、荧光定量PCR检测等实验室诊断方法进行诊断。 结果 临床观察病猪出现食欲不振、体形消瘦、体温升高等症状；病理剖检可见其腹腔内有积液，肠透明充气；肠系膜淋巴结充血；胃溃疡；脾脏边缘坏死。细菌分离培养未长出菌落；荧光定量PCR检测结果为猪伪狂犬病毒、猪圆环病毒2型、猪流行性腹泻病毒有明显的指数增长曲线，口蹄疫病毒、猪瘟病毒、猪轮状病毒、猪传染性胃肠炎病毒无Ct值。 结论 该猪场死亡仔猪存在猪伪狂犬病毒、猪圆环病毒2型、猪流行性腹泻病毒混合感染，主要防控方法为疫苗免疫和提高生物安全防护。

Objectives The cause of abnormal morbidity and mortality in piglets in a pig farming enterprise in Guizhou Province was diagnosed to provide reference for preventing and controlling disease in pig farming enterprises. Methods The heart, liver, spleen, lungs, kidneys, lymph nodes and other tissues from 2 sick piglets aged 3-14 days were collected aseptically. Diagnosis was conducted with laboratory diagnostic methods including clinical observation, pathological dissection, isolation and culture of bacteria, and fluorescence quantitative PCR detection. Results There were symptoms including poor appetite, emaciation, and fever in affected pigs via clinical observation. There was peritonitis with gas accumulation in the intestines, congestion of mesenteric lymph nodes, gastric ulcer, and necrosis of the spleen edge via pathological dissection. No colonies were grown in the isolation and culture of bacteria. The results of fluorescent quantitative PCR detection showed that there was a significant exponential growth curve for porcine pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), and porcine epidemic diarrhea virus (PEDV). No Ct value of foot-and-mouth disease virus, classical swine fever virus, porcine rotavirus, and porcine transmissible gastroenteritis virus was detected. Conclusions The dead piglets in the pig farm were infected with a mixture of PRV, PCV2, and PEDV. The main methods of prevention and control are vaccine immunization and improvement in biosafety protection.