目的 分析中药连花清瘟方剂对2种常见畜禽病原菌（巴氏杆菌、猪丹毒丝菌）的抗菌作用，为其在兽医临床中的应用提供参考。 方法 首先，通过微量肉汤稀释法测定连花清瘟方剂对巴氏杆菌3株不同菌株（巴氏杆菌X7株、巴氏杆菌YU株、巴氏杆菌SD株）的最小杀菌浓度（MBC）和最小抑菌浓度（MIC）。然后，进行药物治疗试验。取4~6周龄小鼠50只，分为5组，巴氏杆菌攻毒后给予各组不同的治疗方案（连花清瘟方剂、无菌水、阿莫西林、头孢克洛）治疗3 d，通过治疗有效率评价连花清瘟方剂对巴氏杆菌感染的治疗效果；参照连花清瘟方剂抗巴氏杆菌的试验方法，测定连花清瘟方剂对猪丹毒丝菌的MIC、MBC和治疗有效率。 结果 连花清瘟方剂对巴氏杆菌不同菌株的MIC和MBC分别为4~8、8~16 mg/mL，对巴氏杆菌感染的治疗有效率为60％，显著高于对照组，与抗生素灌服组治疗无显著差异。连花清瘟方剂对猪丹毒丝菌SE38株的MIC值为64 mg/mL，MBC值为128 mg/mL，小鼠感染模型中无显著治疗效果。 结论 连花清瘟方剂对巴氏杆菌具有较好的抗菌效果，可作为抗菌药替代品进一步研究。

Objectives The antibacterial effects of traditional Chinese medicine Lianhua-Qingwen formula on two common pathogens in livestock and poultry including Pasteurellamultocida and Erysipelothrix rhusiopathiae were studied to provide reference for its application in veterinary clinic. Methods The minimum bactericidal concentration (MBC) and minimum inhibitory concentration (MIC) of Lianhua-Qingwen formula against three different strains of Pasteurella including Pasteurella X7, Pasteurella YU, Pasteurella SD were measured with the microbroth dilution method. The experiment of drug therapy was conducted. 50 mice aged 4-6 weeks were divided into 5 groups. Each group after infection with Pasteurella was treated with different therapeutic regimens including Lianhua-Qingwen formula, sterile water, amoxicillin, cefaclor for 3 days. The therapeutic effect of Lianhua-Qingw formula on Pasteurella infection was evaluated with the efficacy of treatment. The MIC, MBC, and the efficacy of treatment of Lianhua-Qingwen formula against Erysipelothrix rhusiopathiae were determined according to the anti-Pasteurella test method of Lianhua-Qingwen formula. Results The MIC and MBC of Lianhua-Qingwen formula for different strains of Pasteurella was 4-8 mg/mL and 8-16 mg/mL, respectively. The efficacy of treatment for Pasteurella infection was 60%, significantly higher than that in the control group, and there was no significant difference in treatment compared to the antibiotic oral group. The MIC and MBC of Lianhua-Qingwen formula against SE38 strain of Erysipelothrix rhusiopathiae was 64 mg/mL and 128 mg/mL, and there was no significant therapeutic effect in the mouse infection model. Conclusions Lianhua-Qingwen formula has a good antibacterial effect on Pasteurella, and can be can be further studied as a substitute for antibiotics.