目的 为解决云南高原地区鸡精液冷冻保存质量差的问题，优化鸡精液冷冻保存的技术流程。 方法 选用茶花二号和云岭红种公鸡精液为研究对象，对比分析了不同成分的精液稀释液、不同比例的抗冻剂及冷冻方法的保存效果，使用计算机辅助精子分析系统、姬姆萨染色法和伊红染色法检测鸡精液的冻后活力、顶体完整率、畸形率。 结果 基础液配方2液、3液和7液效果较好；GL+10%卵黄和DMSO+20%卵黄的冻精活力显著优于DMA+20%卵黄、EG+15%卵黄，分别为34.1%、31.6%、27.4%、19.9%，精子顶体完整率差异不显著；应用程控仪和大口罐冷冻其精液的冻后活力、顶体完整率、畸形率差异不显著。 结论 应用程控仪采用添加葡萄糖、乳糖及柠檬酸钠为基础液，添加防冻剂EG+15%卵黄和DMSO+20%卵黄制作冷冻精液为佳

Objective The technical process of chicken semen cryopreservation was optimized to solve the poor quality of chicken semen cryopreserved in the Yunnan Plateau regions. Methods The semen of Tea Flower No. 2 and Yunling Red Rooster were used to conduct comparative analyses on the effects of semen diluents with different components, antifreeze agents in different proportions, and methods of freezing on the preservation. The computer-assisted sperm analysis system, Giemsa staining method, and Eosin staining method were used to detect the post-freezing motility of sperm, the integrity rate of sperm acrosome, and the rate of abnormality in chicken semen. Results The effect of formulae 2 liquid, 3 liquid and 7 liquid of basic liquid was better. The post-freezing motility of sperm in the semen froze with GL+10% yolk and DMSO+20% yolk was significantly better than that with DMA+20% yolk and EG+15% yolk, with the rate of 34.1%, 31.6%, 27.4%, and 19.9%, respectively. There was no significant difference in the integrity rate of sperm acrosome. The post-freezing motility of sperm, the integrity rate of sperm acrosome, and the rate of abnormality in semen froze with the programmable instrument and the large-mouthed jar was not significantly different as well. Conclusion It is recommended to use a programmable instrument to prepare frozen semen by adding glucose, lactose, and sodium citrate as the basic liquid solution and EG+15% yolk and DMSO+20% yolk as antifreeze agent. It will provide a theoretical basis for the production and studies of frozen chicken semen in plateau regions.