目的 对化皮刺参（Apostichopus japonicus）病例中的寄生虫进行分离鉴定，分析其致病因素，为刺参盾纤毛虫病诊断和防控提供参考依据。 方法 对辽宁某养殖场送检的化皮刺参表皮、体壁、肌肉、体腔液、呼吸树、肠道进行镜检，将观察到的纤毛虫离体培养，并进行18S rDNA测序。 结果 病参各组织器官观察到纤毛虫H-1、H-2、H-3和H-4，H-4通过形态学判断为后口虫，成功离体培养出H-3与镜检未观察到的H-5。纤毛虫H-3与H-5的18S rDNA序列比对结果显示为海洋尾丝虫（Uronema marinum）。 结论 本病例中刺参各组织寄生多种纤毛虫，体腔液中寄生海洋尾丝虫，呼吸树中寄生后口虫和海洋尾丝虫。

Objectives The parasites in the cases of Apostichopus japonicus were isolated and identified, and its pathogenic factors were analyzed to provide a reference for the diagnosis, prevention and control of scuticociliate in A.japonicus. Methods Microscopic examination was conducted on the epidermis, body wall, muscles, body cavity fluid, respiratory tree, and intestines of the A.japonicus sent from a farm in Liaoning Province for inspection. The observed ciliates were cultured in vitro and its 18S rDNA were sequenced. Results The ciliate H-1, H-2, H-3 and H-4 was observed in various tissues and organs of the diseased ginseng. The ciliate H-4 was identified as a Boveria Stevens based on morphology. The ciliate H-3 and H-5 not observed under microscopy were successfully cultured in vitro. The alignment of the 18S rDNA sequences of ciliates H-3 and H-5 showed that they were U.marinum. Conclusions In this case, various scuticociliate were parasitized in the tissues of A.japonicus, while U.marinum were parasitized in the body cavity fluid, and Boveria Stevens and U.marinum were parasitized in the respiratory trees.