目的 明确甘肃安定区牛羊布鲁氏菌病的感染状况及流行趋势，分析疫情发生的原因和传播途径，为制定针对性的防控措施提供科学依据。 方法 从安定区牛羊规模化养殖场和散养户中随机抽取未进行布病免疫接种的牛羊，2023年1—12月共采集21 010份血清样本，其中羊血清样本为13 284份，牛血清样本为7 726份。对安定区牛羊布鲁氏菌病流行病学进行分析，结合实验室检测，确诊疑似病例。 结果 在不同的养殖场地中，种畜场阳性检出率最低，其中种羊场总阳性检出率为0.11%，种牛场总阳性检出率为0。在1年监测周期内，羊布病在3、9、10月的阳性检出率较高，分别为0.37%、0.36%、0.27%；牛布病在4、8、9月阳性检出率较高，分别为0.28%、0.32%、0.31%。 结论 在春秋季节，牛羊布病更容易流行，因此应从监测净化、消毒灭源、养殖管理等方面入手，采取有效的防控措施，减轻牛羊布病的危害。

Objectives The infection status and epidemic trend of brucellosis in cattle and sheep in Anding District, Gansu Province was studied and the causes and transmission routes of the epidemic were analyzed to provide scientific basis for formulating specific measures for prevention and control. Methods The cattle and sheep having not been vaccinated against brucellosis from large-scale cattle and sheep farms and free-range households in Anding District, Gansu Province in 2023 were randomly selected. A total of 21010 serum samples including 13284 sheep serum samples and 7726 cattle serum samples were collected from January to December 2023. The epidemiology of brucellosis in cattle and sheep in Anding District was analyzed. The suspected cases were confirmed by laboratory testing. Results Among different farming sites, the positive detection rate in breeding farms was the lowest, with a total positive detection rate of 0.11% in sheep breeding farms and 0 in cattle breeding farms. During the period of one-year monitoring, the positive detection rate of brucellosis in sheep was relatively higher in March, September, and October, with rate of 0.37%, 0.36%, and 0.27%, respectively. The positive detection rate of brucellosis in cattle was relatively higher in April, August and September, with rate of 0.28%, 0.32% and 0.31%, respectively. Conclusions It is indicated that the brucellosis in cattle and sheep is more prevalent in the spring and autumn seasons. Therefore, the effective measures for prevention and control should be taken from aspects including monitoring and purification, disinfection and farming management to reduce the harm caused by brucellosis in cattle and sheep.