2021—2023年云南临沧市小反刍兽疫病原学和免疫抗体检测分析

李亚琴, 蔡高萍, 谭东, 杨依波, 杨小芳

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养殖与饲料 ›› 2024, Vol. 23 ›› Issue (05) : 27-30. DOI: 10.13300/j.cnki.cn42-1648/s.2024.05.005
试验研究

2021—2023年云南临沧市小反刍兽疫病原学和免疫抗体检测分析

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Analyzing the pathology and immunological antibody detection of Peste des petits ruminants in Lincang City, Yunnan Province, 2021—2023

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摘要

目的 为全面掌握临沧市小反刍兽疫免疫效果,科学评估其感染状况。 方法 随机采集2021—2023年来自规模场和散养户的羊血清4 524份、羊眼鼻棉拭子1 000份,采用阻断ELISA方法进行小反刍兽疫抗体检测,病毒核酸荧光RT-PCR进行病原学检测。 结果 小反刍兽疫免疫抗体合格率,2022年比2021年提高了7.44百分点,2023年比2022年提高了3.11百分点,病原学未检测出小反刍兽疫病毒核酸阳性样品。 结论 临沧市以“人病兽防、关口前移”各项防控措施为抓手,小反刍兽疫的免疫效果总体较好。

Abstract

Objectives To comprehensively grasp the immune effect of small ruminant plague in Lincang City and scientifically evaluate its infection status. Methods 4 524 serum samples and 1 000 eye and nose cotton swabs of sheep from large-scale farms and free-range households were randomly collected from 2021 to 2023. Blocking ELISA method was used to detect antibodies against Peste des petits ruminants (PPR). The fluorescence RT-PCR of viral nucleic acid was used to detect the pathogen of PPR. Results The qualification rate of immune antibodies against PPR in 2022 was 7.44 percentage points higher than that in 2021, and that in 2023 was 3.11 percentage points higher than that in 2022. No positive samples of PPR virus nucleic acid were detected with pathology. Conclusions Lincang City has focused on various measures of prevention and control including 'prevention human from infecting animal diseases, and moving the keypoints forward', and the immune effect of small ruminant plague is good in general.

关键词

小反刍兽疫 / 抗体检测 / 病原学检测 / 疫苗免疫 / 小反刍兽疫病毒 / 荧光RT-PCR

Key words

Peste des petits ruminants (PPR) / antibody detection / pathological detection / vaccine immunization / peste petit ruminant virus / fluorescent RT-PCR

中图分类号

S852.4

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李亚琴 , 蔡高萍 , 谭东 , . 2021—2023年云南临沧市小反刍兽疫病原学和免疫抗体检测分析. 养殖与饲料. 2024, 23(05): 27-30 https://doi.org/10.13300/j.cnki.cn42-1648/s.2024.05.005
LI Yaqin, CAI Gaoping, TAN Dong, et al. Analyzing the pathology and immunological antibody detection of Peste des petits ruminants in Lincang City, Yunnan Province, 2021—2023[J]. Animals Breeding and Feed. 2024, 23(05): 27-30 https://doi.org/10.13300/j.cnki.cn42-1648/s.2024.05.005

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