
大豆低聚肽对嗜酸乳杆菌的增殖效果研究
高建胜
大豆低聚肽对嗜酸乳杆菌的增殖效果研究
Effects of soybean oligopeptides on the proliferation of Lactobacillus acidophilus
目的 探究大豆低聚肽对嗜酸乳杆菌生长的影响,为乳酸菌体内定植提供思路。 方法 试验组加入不同质量浓度的大豆低聚肽于MRS肉汤培养基中,培养嗜酸乳杆菌48 h测定OD600 nm;测定不同培养时间下嗜酸乳杆菌培养液的OD600 nm,绘制生长曲线;以300、600、1 200 mg/kg剂量的大豆低聚肽-嗜酸乳杆菌菌悬液连续灌胃抗生素诱导的肠道菌群紊乱小鼠7 d,测定大豆低聚肽作用下小鼠体质量变化。 结果 40、30、20 mg/mL大豆低聚肽能明显增加嗜酸乳杆菌菌液浊度,促进嗜酸乳杆菌增殖;能使嗜酸乳杆菌培养12 h后进入对数生长期,提前进入快速增长状态并提高增长速度;低剂量组(300 mg/kg)、中剂量组(600 mg/kg)、高剂量组(1 200 mg/kg)均能促进小鼠体质量增长,且600 mg/kg中剂量组体质量增加2.89 g,与阴性对照组差异显著。 结论 大豆低聚肽在体外能明显促进嗜酸乳杆菌增殖,添加的大豆低聚肽质量浓度为40 mg/mL时增殖效果最为显著。能促进小鼠体质量增长,但其促进乳酸菌的体内定植机制需要进一步评价。
Objectives The effects of soybean oligopeptides on the growth of Lactobacillus acidophilus were studied to provide ideas for the colonization in vivo of Lactobacillus spp. Method Different concentrations of soy oligopeptides were added to the MRS broth medium in the experimental group, and Lactobacillus acidophilus was cultured for 48 hours to measure OD600 nm. The OD600 nm of Lactobacillus acidophilus culture solution at different cultured times was measured to plot the growth curve. Mice with intestinal microbiota disorder induced by antibiotics were continuously gavaged with soybean oligopeptides-Lactobacillus acidophilus suspension at doses of 300, 600, and 1 200 mg/kg for 7 days. The changes in body weight of mice under the action of soybean oligopeptides were determined. Results 40, 30, and 20 mg/mL soy oligopeptides significantly increased the turbidity of Lactobacillus acidophilus, promoted its proliferation, enabled Lactobacillus acidophilus to enter a logarithmic growth phase after 12 hours of culture and enter a rapid growth state in advance, and increased the growth rate. The low-dose group of 300 mg/kg, the medium dose group of 600 mg/kg, and the high-dose group of 1 200 mg/kg all promoted the weight gain of mice. The body weight of the medium dose group of 600 mg/kg increased by 2.89 g, which was significantly different from the control group. Conclusions Soybean oligopeptides can significantly promote the proliferation in vitro of Lactobacillus acidophilus, and the proliferation effect is most significant when the concentration of soybean oligopeptides added is 40 mg/mL. Adding soybean oligopeptides can promote the weight gain of mice, but its mechanism of promoting the colonization in vivo of Lactobacillus acidophilus needs further evaluation.
大豆低聚肽 / 嗜酸乳杆菌 / 体外增殖 / 体内定植 / 肠道营养
soybean oligopeptides / Lactobacillus acidophilus / proliferation in vitro / colonization in vivo / intestinal health
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