目的 了解新疆师市部分规模牛场疫病发病原因及流行情况，揭示致病原因，为制定防控措施奠定基础。 方法 选择师市范围内19个规模牛场，共采集健康牛血清样品2 923份，鼻拭子1 127份，分别进行传染性牛鼻气管炎(IBR)实时荧光PCR检测和传染性牛鼻气管炎ELISA方法检测抗原和抗体。 结果 实时荧光PCR检测抗原总阳性率为1.69%，其中：荷牛阳性率为1.83%，弗荷牛阳性率为1.57%，挪荷牛阳性率为1.66%；场群阳性率为78.95%。3个品系的犊牛、青年牛、后备牛、成母牛均检测到IBRV，不同品系牛之间不影响IBR的流行，3个品系牛之间抗原阳性率差异不显著。牛传染性鼻气管炎ELISA方法检测抗体总阳性率为53.71%，其中：荷牛阳性率为54.82%，弗荷牛阳性率为53.86%，挪荷牛阳性率为52.30%，场群抗体阳性率为100%，3个品系牛之间抗体阳性率差异不显著。 结论 师市模化牛场3个品系牛均存在一定程度的传染性牛鼻气管炎病毒(IBRV)感染。

Objectives The causes and prevalence of diseases in some large-scale cattle farms in Shihezi City, Xinjiang were understood and the causes of pathogenesis were revealed to lay the foundation for formulating measures of prevention and control. Methods A total of 2 923 healthy bovine serum samples and 1 127 nasal swabs were collected from 19 large-scale cattle farms within Shihezi City. The infectious bovine rhinotracheitis (IBR) was detected with real-time fluorescence PCR and the antigens and antibodies for IBR were detected with ELISA. Results The total positive rate of real-time fluorescent PCR for antigen detection was 1.69%, with the positive rate of Dutch cattle, Feho cattle, Noho cattle, and the farm herd being 1.83%, 1.57%, 1.66%, and 78.95%, respectively. IBRV was detected in calves, young cattle, reserve cattle, and adult cows of three lines, and the prevalence of IBR was not affected among different cattle lines, indicating that there was no significant difference in the positive rate of antigen among the three cattle breeds. The total positive rate of antibodies for IBR detected by ELISA was 53.71%. Among them, the positive rate of Dutch cattle, Feho cattle, Noho cattle, and the farm herd was 54.82%, 53.86%, 52.30%, and 100%, respectively. There was no significant difference in the positive rate of antibodies among the three cattle breeds. Conclusions All three cattle breeds in the large-scale cattle farms in Shihezi City had a certain degree of infectious bovine rhinotracheitis virus (IBRV) infection.