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血浆无细胞线粒体外线粒体DNA与牙周炎临床指标的相关性研究
杨再目,曹沛,刘振华,栾庆先
PDF(1836 KB)
PDF(1836 KB)
血浆无细胞线粒体外线粒体DNA与牙周炎临床指标的相关性研究
),曹沛1,刘振华2,栾庆先1()Correlation study of plasma cell-free extra-mitochondrial mitochondria DNA and periodontitis clinical parameters
),Pei Cao1,Zhenhua Liu2,Qingxian Luan1()目的 血浆无细胞线粒体外线粒体DNA（cf-exmtDNA）具有促炎潜能，本文探讨血浆cf-exmtDNA与牙周炎临床指标的相关性。 方法 纳入18~45岁受试者78人，其中牙周健康者11人，牙龈炎患者11人，牙周炎患者56人。检查并记录基线牙周指标、年龄、性别、体质指数（BMI）和空腹血糖（FBG）。取4 mL抗凝静脉血，采用二次离心法提取其中cf-exmtDNA，使用实时荧光定量聚合酶链式反应检测cf-exmtDNA拷贝数。比较不同牙周炎症状态组血浆cf-exmtDNA水平，并对血浆cf-exmtDNA与平均探诊深度（mPD）、平均附着水平（mCAL）、平均出血指数（mBI）、平均菌斑指数（mPLI）、年龄、FBG、BMI等指标进行相关性分析以及多重线性回归分析。 结果 牙周炎组血浆cf-exmtDNA水平显著高于牙周健康组（P=0.042）；样本整体血浆cf-exmtDNA水平与年龄（P=0.023）、mPD（P<0.001）、mCAL（P=0.006）、mBI（P=0.026）呈正相关关系；多重回归分析中，血浆cf-exmtDNA水平主要取决于mPD。 结论 在18~45岁人群中，牙周炎患者血浆cf-exmtDNA水平较牙周健康者显著升高，血浆cf-exmtDNA水平与年龄、mPD、mCAL、mBI呈正相关关系。
Objective Plasma cell-free extra-cellular mitochondrial DNA (cf-exmtDNA) shows pro-inflammatory potential. This study aims to investigate the correlation between cf-exmtDNA and baseline periodontitis clinical parameters in patients in general health. Methods A total of 78 participants aged 18-45 years were enrolled: 11 periodontal healthy volunteers, 11 patients with gingivitis, and 56 patients with periodontitis. Baseline periodontal index, age, gender, body mass index (BMI), and baseline fasting blood glucose (FBG) were examined and recorded. Anticoagulated venous blood (4 mL) was collected, and cf-mtDNA was extracted by secondary centrifugation. cf-exmtDNA concentration was measured through real-time quantitative polymerase chain reaction. Different states of periodontal inflammation were compared in terms of plasma cf-exmtDNA copy number, and the relationship among plasma cf-exmtDNA, mean probing depth (mPD), mean clinical attachment level (mCAL), mean bleeding index (mBI), mean plaque index, age, FBG, and BMI were examined through correlation and multiple linear regression analyses. Results Patients with periodontitis had significantly higher plasma cf-exmtDNA levels than the healthy volunteers (P=0.042). The overall plasma cf-exmtDNA was significantly positively correlated with age (P=0.023), mPD (P<0.001), mCAL (P=0.006), and mBI (P=0.026). Multiple regression analysis showed that plasma cf-exmtDNA level was significantly dependent on mPD. Conclusion In the general population aged 18–45, patients with periodontitis had significantly higher plasma cf-exmt-DNA level than the healthy volunteers, and plasma cf-exmtDNA concentration was significantly positively correlated with age, mPD, mCAL, and mBI.
牙周炎 / 无细胞DNA / 线粒体DNA / 横断面研究 / 牙周临床指标
periodontitis / cell-free DNA / mitochondrial DNA / cross-sectional study / periodontal clinical parameters
R781.4
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