Pharmacology experiment is highly intellectual, practical, exploratory and creative, emphasizing the cultivation of students’ comprehensive abilities. However, in traditional experimental teaching, students overly rely on teachers' instructional slides explanation and demonstrations, and lack in-depth thinking about experiments, which makes it difficult to achieve the expected results. To address this issue, the reform takes students of two cohorts in pharmaceutical chemistry major as the research object, adopts the "5E" teaching model, guided by interest and inquiry, integrates scientific research application with experimental teaching based on the original teaching plan, sets up PAD class, guides students to actively think and discuss problems, makes full use of online resources, encourages students to study independently, and expands their knowledge. After implementing these reforms, a questionnaire survey and ability assessment were conducted to evaluate student outcomes. The final results demonstrated that the reform significantly improved students’ learning experience, particularly in scientific thinking and hands-on skills.