Objective To explore a new model of online-offline and in class-out of class formative evaluation of analysis of traditional Chinese materia medica. Method The study carried out teaching practice on 64 students majored in traditional Chinese medicine in grade 2019. Based on the examination mode of formative evaluation by rain classroom, the teaching process and the results were analyzed after the course. Result Through multi-mode examinations such as online out-of-class usual tests, offline in-class flipped classroom, offline out-of-class semester assignments and offline final exams, the mastery and application of professional knowledge as well as the abilities of language expression and paper writing have been improved. Conclusion A new model of online-offline and in class-out of class formative evaluation of analysis of traditional Chinese materia medica was carried out. The students' comprehensive abilities have been improved. The evaluation of students' learning effect was more comprehensive, objective and fair. And the teachers improved teaching methods and contents constantly.