Course learning is an essential component of postgraduate education. Due to the unique nature of pharmaceuticals, which are subject to strict legal regulations, industry guidelines should be incorporated into pharmacy education, especially in postgraduate programs. Taking the graduate course (PR5211 Pharmaceutical Analysis IV) at the National University of Singapore (NUS) as an example, this paper discusses how to design a postgraduate course in Biopharmaceutical Analysis based on the guidance for industry: Bioanalytical Method Validation which is issued by regulatory authorities. This course takes industry guidelines as its framework, teaches pharmaceutical analysis knowledge in real-world case studies, and assesses the application of analytical skills in actual scenarios. It particularly emphasizes the cultivation of students' lifelong learning abilities and has achieved good results in practice. This educational model is worth adhering to and promoting in other graduate courses in pharmacy.