Through analyzing the characteristics of the Analytical Chemistry and the current situation of differentiated teaching, this paper discusses the design and practice of differentiated teaching reform in Analytical Chemistry from the aspects of teaching teams, design of teaching content and form, process evaluation and the realization of teaching design under the background of reduced class hours. Differentiated teaching reform in Analytical Chemistry should focus on “basic knowledge + professional atmosphere” and “online and offline mixed teaching”. It enables students to progressively learn theories and consolidate and expand professional knowledge under the guidance of course learning process maps, thereby establish confidence in learning and professional interests. Regarding corresponding process evaluation, the evaluation mode adopted can promote students to achieve their own high standards and levels, achieving the purpose of evaluation.