Organic Chemistry is the main basic course for students major in chemistry, applied chemistry and pharmacy. In the 21st century, the teaching goals of organic chemistry are not only to impart professional basic knowledge, but also to instill the idea of quality education and cultivate students' ability to acquire knowledge and innovate. Therefore, based on the reform of the curriculum content and system and combined with the use of multimedia teaching methods, "One Center, Four Strengthening" teaching method is proposed to improve the "thickness" and "depth" of the course. Results and conclusions Through a series of teaching reforms, students' initiative in learning has been greatly stimulated, their academic performance has been greatly improved, and their awareness of innovation has been significantly enhanced. This study provides a reference for the improvement of teaching methods in organic chemistry for pharmaceutical majors in universities.