Application of platelet-rich plasma combined with Xianling Gubao Capsule in treatment of rats with knee osteoarthritis

Cai Zhenhua, Xiao Muzhou, Luo Heng, Wu Zhilan, Li Li, Lü Cong

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Journal of Chongqing Medical University ›› 2025, Vol. 50 ›› Issue (05) : 623-629. DOI: 10.13406/j.cnki.cyxb.003811
Basic research

Application of platelet-rich plasma combined with Xianling Gubao Capsule in treatment of rats with knee osteoarthritis

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Abstract

Objective To investigate the therapeutic effect of platelet-rich plasma（PRP） combined with Xianling Gubao Capsule on rats with osteoarthritis（OA） and the changes in the levels of related factors. Methods Anterior cruciate ligament transection was performed to establish a rat model of OA，and after modeling，the rats were randomly divided into control group，model group，Xianling Gubao Capsule group，PRP group，and Xianling Gubao Capsule+PRP group，with 10 rats in each group. After intervention，ELISA was used to measure the serum levels of IL-1β and TNF-α； knee articular cartilage was collected，and qPCR and Western blot were used to measure the mRNA and protein expression levels of MMP3，MMP13，Col2，Aggrecan，and p-p38； Micro-CT was used to assess the changes in bones around the knee joint； HE staining and SF staining were used to observe the changes in knee articular cartilage. Results ELISA showed that PRP combined with Xianling Gubao Capsule significantly reduced the serum levels of the inflammatory factors such as IL-1β and TNF-α（P<0.05）. The results of qPCR and Western blot showed that PRP combined with Xianling Gubao Capsule significantly reduced the expression levels of MMP3，MMP13，and p-p38 and increased the expression levels of Col2 and Aggrecan in knee articular cartilage（P<0.05）. Micro-CT，HE staining，and SF staining showed a certain degree of improvement in knee articular cartilage degeneration in the Xianling Gubao Capsule+PRP group （P<0.05）. Conclusion PRP combined with Xianling Gubao Capsule exerts a therapeutic effect on rats with OA，possibly by regulating the p38MAPK signaling pathway.

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osteoarthritis / platelet-rich plasma / Xianling Gubao Capsule / p38 mitogen-activated protein kinase

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Cai Zhenhua , Xiao Muzhou , Luo Heng , et al . Application of platelet-rich plasma combined with Xianling Gubao Capsule in treatment of rats with knee osteoarthritis. Journal of Chongqing Medical University. 2025, 50(05): 623-629 https://doi.org/10.13406/j.cnki.cyxb.003811

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