Study of circTRRAP knockdown in acute myocardial infarction by regulating miR-323-3p/SMAD2 axis

Xiong Yan, Tang Yijia

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Journal of Chongqing Medical University ›› 2025, Vol. 50 ›› Issue (04) : 547-556. DOI: 10.13406/j.cnki.cyxb.003788
Clinical research

Study of circTRRAP knockdown in acute myocardial infarction by regulating miR-323-3p/SMAD2 axis

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Abstract

Objective To investigate the specific role of circTRRAP in acute myocardial infarction（AMI）. Methods The method of hypoxia for 24 hours was used to induce the model of myocardial infarction，and the dual-luciferase reporter assay was used to investigate the interaction between circTRRAP，SMAD2，and miR-323-3p. After knockdown and overexpression of miR-323-3p and overexpression of SMAD2 through transfection with si-circTRRAP，the expression levels of proinflammatory cytokines ［interkeukin-6（IL-6） and tumor necrosis factor-α（TNF-α）］，oxidative stress markers ［malondialdehyde（MDA） and superoxide dismutase（SOD）］，and apoptotic factors［Bcl-2-associated X protein（Bax），B-cell lymphoma-2（Bcl-2），and cleaved caspase-3］were measured to investigate the role of circTRRAP，SMAD2，and miR-323-3p in myocardial infarction. Results In the model of myocardial infarction injury，the levels of circTRRAP and SMAD2 were significantly increased by more than 50%，whereas there was a significant reduction in the expression of miR-323-3p. The downregulation of circTRRAP led to a reduction in SMAD2 expression by promoting miR-323-3p expression. SMAD2 was negatively correlated with miR-323-3p，but it was positively correlated with the expression of circTRRAP. The downregulation of circTRRAP or SMAD2 or the upregulation of miR-323-3p could increase cell viability and reduce the apoptosis rate of cardiomyocytes. Conclusion Downregulation of circTRRAP can inhibit inflammation and alleviate AMI via the miR-323-3p/SMAD2 axis.

Key words

circular RNA / gene regulation / cell apoptosis / myocardial infarction

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Xiong Yan , Tang Yijia. Study of circTRRAP knockdown in acute myocardial infarction by regulating miR-323-3p/SMAD2 axis. Journal of Chongqing Medical University. 2025, 50(04): 547-556 https://doi.org/10.13406/j.cnki.cyxb.003788

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