Objective To establish a mouse model of spinal cord injury （SCI），and to investigate the effect of electroacupuncture（EA） intervention on cell apoptosis after acute SCI and its mechanism. Methods Female C57BL/6 mice were used to establish a model of SCI，and after successful modeling，the mice were randomly divided into SCI group，EA group，and Rosiglitazone group（R group）；a sham-operation group（Sham group） was also established. After successful modeling，the mice in the EA group were given EA at bilateral Jiaji points and Zusanli once a day for 14 days，those in the R group were given intraperitoneal injection of the PPARγ agonist Rosiglitazone（5 mg/kg），and those in the Sham group and the SCI group were not given any specific treatment. BMS score was used to assess motor function；HE staining and immunofluorescence assay were used to observe pathological changes after SCI；RNA-Seq and TMT/iTRAQ techniques were used to identify the targets and mechanisms of EA；Western blot was used to measure the protein expression levels of PPARγ，CD36，Hba-a1，Hbb-bt，and caspase-3. Results Compared with the SCI group，the EA group and the R group had improvements in hindlimb motor function，tissue structure，and the number of surviving cells. The EA group and the R group had a significant reduction in the expression of caspase-3 and significant increases in the expression of PPARγ and CD36，and the EA group had significant reductions in the expression of Hba-a1 and Hbb-bt. In addition，RNA-Seq and TMT/iTRAQ techniques，significant analysis，Venn analysis，and dual-omics analysis identified Hba-a1 and Hbb-bt as the target genes of EA. The KEGG pathway enrichment analysis showed that EA had a significant effect on the PPAR signaling pathway. Conclusion By regulating the PPARγ-CD36 signaling pathway，EA can promote the clearance of Hba-a1 and Hbb-bt after SCI，reduce the expression level of caspase-3，alleviate cell apoptosis，and facilitate the recovery of spinal cord nerve function.