Objective To assess the efficacy and safety of a novel domestic single-port robotic surgical system for lobectomy through an animal experiment，and to lay the groundwork for its clinical application. Methods An experimental Large White pig （male，40 kg） was selected to undergo left lower lobectomy and systematic lymph node dissection under the assistance of the SHURUI^® single-port surgical robot. Its feasibility，safety，and efficacy during the perioperative period were evaluated. Results Left lower lobectomy and systematic lymph node dissection were performed successfully with the single-port robotic surgical system with snake-like arms. The total operative time was 115 min，the docking time was 5 min，the operative time inside the chest cavity was 100 min，the time for lobectomy was 85 min，and the time for lymph node dissection was 15 min. The blood loss was about 20 mL. During operation，there was no damage to the lung，pericardium，vessels，or nerves；no conversion to open surgery； no interference or clash between instruments or instance of instruments moving away from the field of vision； no injuries of the vessels，lung，or chest wall caused by blind operation；and no system failure. The pig showed stable vital signs during and after the operation，and recovered well without relevant complications after the surgery. Conclusion The SHURUI^® single-port robotic surgical system is safe and feasible for performing lobectomy in pigs.