Advances in nucleic acid detection methods for hepatitis B virus

Li Yalan, Ma Yuanyan, Wang Deqiang, Cai Xuefei

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Journal of Chongqing Medical University ›› 2025, Vol. 50 ›› Issue (02) : 152-157. DOI: 10.13406/j.cnki.cyxb.003654
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Advances in nucleic acid detection methods for hepatitis B virus

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Abstract

Hepatitis B virus infection is a major public health problem.To avoid the development of liver cirrhosis,liver failure,and hepatocellular carcinoma,patients usually need long-term drug treatment. The dynamic change in serum hepatitis B virus nucleic acid levels can be used as an important indicator of safe drug withdrawal in clinical practice. For the precise treatment of hepatitis B,it is very important to improve the specificity and sensitivity of hepatitis B virus nucleic acid detection methods and the scientific interpretation of the results. This paper summarizes the application scenarios of existing detection methods and their advantages and disadvantages,aiming to provide a reference for method selection according to clinical situation in the future.

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nucleic acid detection / hepatitis B virus / research progress

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Li Yalan , Ma Yuanyan , Wang Deqiang , et al. Advances in nucleic acid detection methods for hepatitis B virus. Journal of Chongqing Medical University. 2025, 50(02): 152-157 https://doi.org/10.13406/j.cnki.cyxb.003654

References

1
Jeng WJ Papatheodoridis GV Lok ASF. Hepatitis B[J]. Lancet2023401(10381):1039-1052.
2
Thomas DL. Global elimination of chronic hepatitis[J]. N Engl J Med2019380(21):2041-2050.
3
Liu J Liang WN Jing WZ,et al. Countdown to 2030:eliminating hepatitisB disease,China[J]. Bull World Health Organ201997(3):230-238.
4
Wong GLH Gane E Lok ASF. How to achieve functional cure of HBV:stopping NUCs,adding interferon or new drug development?[J]. J Hepatol202276(6):1249-1262.
5
Ghany MG Buti M Lampertico P,et al. Guidance on treatment endpoints and study design for clinical trials aiming to achieve cure in chronic hepatitis B and D:report from the 2022 AASLD-EASL HBV-HDV Treatment Endpoints Conference[J]. J Hepatol202379(5):1254-1269.
6
Nassal M. HBV cccDNA:viral persistence reservoir and key obstacle for a cure of chronic hepatitis B[J]. Gut201564(12):1972-1984.
7
Karayiannis P. Hepatitis B virus:virology,molecular biology,life cycle and intrahepatic spread[J]. Hepatol Int201711(6):500-508.
8
Inoue T Tanaka Y. The role of hepatitis B core-related antigen[J]. Genes201910(5):357.
9
Freymuth F Eugene G Vabret A,et al. Detection of respiratory syncytial virus by reverse transcription-PCR and hybridization with a DNA enzyme immunoassay[J]. J Clin Microbiol199533(12):3352-3355.
10
Mullis KB Faloona FA. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction[J]. Methods Enzymol1987155:335-350.
11
Kidd IM Clark DA Emery VC. A non-radioisotopic quantitative competitive polymerase chain reaction method:application in measurement of human herpesvirus 7 load[J]. J Virol Methods200087(1/2):177-181.
12
Heid CA Stevens J Livak KJ,et al. Real time quantitative PCR[J]. Genome Res19966(10):986-994.
13
Kubista M Andrade JM Bengtsson M,et al. The real-time polymerase chain reaction[J]. Mol Aspects Med200627(2/3):95-125.
14
Abe A Inoue K Tanaka T,et al. Quantitation of hepatitis B virus genomic DNA by real-time detection PCR[J]. J Clin Microbiol199937(9):2899-2903.
15
Loeb KR Jerome KR Goddard J,et al. High-throughput quantitative analysis of hepatitis B virus DNA in serum using the TaqMan fluorogenic detection system[J]. Hepatology200032(3):626-629.
16
Kusumoto S Tanaka Y Suzuki R,et al. Monitoring of hepatitis B virus (HBV) DNA and risk of HBV reactivation in B-cell lymphoma:a prospective observational study[J]. Clin Infect Dis201561(5):719-729.
17
Yu GX Chen R Zheng SJ,et al. A standardized assay for the quantitative detection of serum HBV RNA in chronic hepatitis B patients[J]. Emerg Microbes Infect202211(1):775-785.
18
Sykes PJ Neoh SH Brisco MJ,et al. Quantitation of targets for PCR by use of limiting dilution[J]. Biotechniques199213(3):444-449.
19
Lo YM Lun FM Chan KC,et al. Digital PCR for the molecular detection of fetal chromosomal aneuploidy[J]. Proc Natl Acad Sci USA2007104(32):13116-13121.
20
Sanders R Huggett JF Bushell CA,et al. Evaluation of digital PCR for absolute DNA quantification[J]. Anal Chem201183(17):6474-6484.
21
Pomari E Piubelli C Perandin F,et al. Digital PCR:a new technology for diagnosis of parasitic infections[J]. Clin Microbiol Infect201925(12):1510-1516.
22
Rački N Dreo T Gutierrez-Aguirre I,et al. Reverse transcriptase droplet digital PCR shows high resilience to PCR inhibitors from plant,soil and water samples[J]. Plant Methods201410(1):42.
23
Taylor SC Carbonneau J Shelton DN,et al. Optimization of Droplet Digital PCR from RNA and DNA extracts with direct comparison to RT-qPCR:clinical implications for quantification of Oseltamivir-resistant subpopulations[J]. J Virol Methods2015224:58-66.
24
Taylor SC Nadeau K Abbasi M,et al. The ultimate qPCR experiment:producing publication quality,reproducible data the first time[J]. Trends Biotechnol201937(7):761-774.
25
Taylor SC Laperriere G Germain H. Droplet Digital PCR versus qPCR for gene expression analysis with low abundant targets:from variable nonsense to publication quality data[J]. Sci Rep20177(1):2409.
26
Caviglia GP Abate ML Tandoi F,et al. Quantitation of HBV cccDNA in anti-HBc-positive liver donors by droplet digital PCR:a new tool to detect occult infection[J]. J Hepatol201869(2):301-307.
27
Limothai U Chuaypen N Poovorawan K,et al. Reverse transcriptase droplet digital PCR vs reverse transcriptase quantitative real-time PCR for serum HBV RNA quantification[J]. J Med Virol202092(12):3365-3372.
28
Deng HM Gao ZQ. Bioanalytical applications of isothermal nucleic acid amplification techniques[J]. Anal Chim Acta2015853:30-45.
29
Yan L Zhou J Zheng Y,et al. Isothermal amplified detection of DNA and RNA[J]. Mol Biosyst201410(5):970-1003.
30
Craw P Balachandran W. Isothermal nucleic acid amplification technologies for point-of-care diagnostics:a critical review[J]. Lab Chip201212(14):2469-2486.
31
Connelly JT Nugen SR Borejsza-Wysocki W,et al. Human pathogenic Cryptosporidium species bioanalytical detection method with single oocyst detection capability[J]. Anal Bioanal Chem2008391(2):487-495.
32
Asiello PJ Baeumner AJ. Miniaturized isothermal nucleic acid amplification,a review[J]. Lab Chip201111(8):1420-1430.
33
Walker GT Fraiser MS Schram JL,et al. Strand displacement amplification:an isothermal,in vitro DNA amplification technique[J]. Nucleic Acids Res199220(7):1691-1696.
34
Liu DY Daubendiek SL Zillman MA,et al. Rolling circle DNA synthesis:small circular oligonucleotides as efficient templates for DNA polymerases[J]. J Am Chem Soc1996118(7):1587-1594.
35
Notomi T Okayama H Masubuchi H,et al. Loop-mediated isothermal amplification of DNA[J]. Nucleic Acids Res200028(12):E63.
36
Piepenburg O Williams CH Stemple DL,et al. DNA detection using recombination proteins[J]. PLoS Biol20064(7):e204.
37
Crannell ZA Castellanos-Gonzalez A Irani A,et al. Nucleic acid test to diagnose cryptosporidiosis:lab assessment in animal and patient specimens[J]. Anal Chem201486(5):2565-2571.
38
Hill-Cawthorne GA Hudson LO El Ghany MF,et al. Recombinations in staphylococcal cassette chromosome mec elements compromise the molecular detection of methicillin resistance in Staphylococcus aureus [J]. PLoS One20149(6):e101419.
39
Sakai K Trabasso P Moretti ML,et al. Identification of fungal pathogens by visible microarray system in combination with isothermal gene amplification[J]. Mycopathologia2014178(1/2):11-26.
40
Zhang SL Ravelonandro M Russell P,et al. Rapid diagnostic detection of plum pox virus in Prunus plants by isothermal AmplifyRP(®) using reverse transcription-recombinase polymerase amplification[J]. J Virol Methods2014207:114-120.
41
Daher RK Stewart G Boissinot M,et al. Recombinase polymerase amplification for diagnostic applications[J]. Clin Chem201662(7):947-958.
42
Lobato IM O’Sullivan CK. Recombinase polymerase amplification:basics,applications and recent advances[J]. Trends Analyt Chem201898:19-35.
43
Kelly KF. Southern blotting[J]. Proc Nutr Soc199655(1B):591-597.
44
Cai DW Nie H Yan R,et al. A southern blot assay for detection of hepatitis B virus covalently closed circular DNA from cell cultures[J]. Methods Mol Biol20131030:151-161.
45
Hayes PC Wolf CR Hayes JD. Blotting techniques for the study of DNA,RNA,and proteins[J]. BMJ1989299(6705):965-968.
46
Li XL Zhao JH Yuan Q,et al. Detection of HBV covalently closed circular DNA[J]. Viruses20179(6):139.
47
Zhang H Tu T. Approaches to quantifying hepatitis B virus covalently closed circular DNA[J]. Clin Mol Hepatol202228(2):135-149.
48
Trayhurn P. Northern blotting[J]. Proc Nutr Soc199655(1B):583-589.
49
Hunt EA Broyles D Head T,et al. MicroRNA detection:current technology and research strategies[J]. Annu Rev Anal Chem20158:217-237.
50
Ramkissoon SH Mainwaring LA Sloand EM,et al. Nonisotopic detection of microRNA using digoxigenin labeled RNA probes[J]. Mol Cell Probes200620(1):1-4.
51
Kim SW Li ZH Moore PS,et al. A sensitive non-radioactive northern blot method to detect small RNAs[J]. Nucleic Acids Res201038(7):e98.
52
Pall GS Codony-Servat C Byrne J,et al. Carbodiimide-mediated cross-linking of RNA to nylon membranes improves the detection of siRNA,miRNA and PiRNA by northern blot[J]. Nucleic Acids Res200735(8):e60.
53
Válóczi A Hornyik C Varga N,et al. Sensitive and specific detection of microRNAs by northern blot analysis using LNA-modified oligonucleotide probes[J]. Nucleic Acids Res200432(22):e175.
54
Dong HF Lei JP Ding L,et al. MicroRNA:function,detection,and bioanalysis[J]. Chem Rev2013113(8):6207-6233.
55
Jia Y, Tian C, Wang H, et al. Long non-coding RNA NORAD/miR-224-3p/MTDH axis contributes to CDDP resistance of esophageal squamous cell carcinoma by promoting nuclear accumulation of β-catenin[J]. Mol Cancer202120(1):162-182.
56
Hu XH Zhao LW Ou MR,et al. Evaluation of reverse transcription-polymerase chain reaction and simultaneous amplification and testing for quantitative detection of serum hepatitis B virus RNA[J]. Heliyon20239(8):e18557.

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