Imaging mass cytometry technology and its application in tumor research

Huang Yiwei, Xiang Tingxiu, Liu Xinghe, Ran Jing, Zhao Yi

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Journal of Chongqing Medical University ›› 2025, Vol. 50 ›› Issue (01) : 6-13. DOI: 10.13406/j.cnki.cyxb.003639
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Imaging mass cytometry technology and its application in tumor research

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Abstract

Multiplex single-cell proteomics technology has become a hot topic in biomedical research，among which imaging mass cytometry（IMC） completely solves the serious problem of cross-color between fluorophores and makes up for the lack of tissue spatial information in single-cell sequencing technology. This technique can label dozens of targets simultaneously on a single tissue section，obtain their expression levels and cell localization at the single-cell level，perform in-depth cell phenotypic in situ analysis，and visually depict single-cell proteome maps from the temporal and spatial levels，and due to its unique technical advantages，it has become a powerful tool in the field of tumor research. This article elaborates on the technical principle of IMC and summarizes the advances in the application of IMC in cell phenotype identification，biomarker detection，tumor immunomodulatory determination，tumor heterogeneity differentiation，clinical prognosis guidance，and response prediction by analyzing recent cases，so as to promote the further integration of tumor spatial omics with existing technologies.

Key words

imaging mass cytometry / spatial proteome / single-cell analysis / tumor microenvironment / tumor markers

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Huang Yiwei , Xiang Tingxiu , Liu Xinghe , et al . Imaging mass cytometry technology and its application in tumor research. Journal of Chongqing Medical University. 2025, 50(01): 6-13 https://doi.org/10.13406/j.cnki.cyxb.003639

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