Research progress on intestinal barrier function assessment techniques

Luo Fangli, Sun Luqiang, Wang Siqi, Hou Yujun, Li Ying, Zhou Siyuan

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Journal of Chongqing Medical University ›› 2025, Vol. 50 ›› Issue (01) : 23-29. DOI: 10.13406/j.cnki.cyxb.003635
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Research progress on intestinal barrier function assessment techniques

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Abstract

Intestinal barrier function is very important for overall health. Under physiological conditions，intestinal barrier can effectively prevent the internal environment from being affected by harmful substances and ensure the stability of the internal environment. When the internal environment of the body is damaged by various factors，the intestinal barrier function can be impaired，leading to increased intestinal permeability and thus a series of pathological changes such as intestinal flora shift and endotoxin absorption. By searching，sorting，and summarizing relevant literature，the authors found that there are many methods for detection and evaluation of intestinal barrier function. Indicators measured using blood，urine，stool，or intestinal tissue samples have been used to evaluate intestinal barrier function both in vivo and in vitro. This paper summarizes the methods of intestinal barrier function evaluation，and analyzes the advantages and disadvantages of each evaluation method，in order to provide a reference for the subsequent research in this field.

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intestinal barrier function / evaluation technique / research progress

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Luo Fangli , Sun Luqiang , Wang Siqi , et al . Research progress on intestinal barrier function assessment techniques. Journal of Chongqing Medical University. 2025, 50(01): 23-29 https://doi.org/10.13406/j.cnki.cyxb.003635

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
1
Stalla FM， Astegiano M， Ribaldone DG，et al. The small intestine：barrier，permeability and microbiota[J]. Minerva Gastroenterol，2022，68（1）：98-110.
本文引用 [1]
2
Schoultz I， Keita ÅV. The intestinal barrier and current techniques for the assessment of gut permeability[J]. Cells，2020，9（8）：1909.
本文引用 [3]
3
Martel J， Chang SH， Ko YF，et al. Gut barrier disruption and chronic disease[J]. Trends Endocrinol Metab，2022，33（4）：247-265.
本文引用 [2]
4
González-González M， Díaz-Zepeda C， Eyzaguirre-Velásquez J，et al. Investigating gut permeability in animal models of disease[J]. Front Physiol，2018，9：1962.
本文引用 [1]
5
Drabińska N， Jarocka-Cyrta E. Crosstalk between resveratrol and gut barrier：a review[J]. Int J Mol Sci，2022，23（23）：15279.
本文引用 [1]
6
Citi S. Intestinal barriers protect against disease[J]. Science，2018，359（6380）：1097-1098.
本文引用 [1]
7
Pellegrini C， Fornai M， D’Antongiovanni V，et al. The intestinal barrier in disorders of the central nervous system[J]. Lancet Gastroenterol Hepatol，2023，8（1）：66-80.
8
di Tommaso N， Gasbarrini A， Ponziani FR. Intestinal barrier in human health and disease[J]. Int J Environ Res Public Health，2021，18（23）：12836.
9
Santilli A， Stefanopoulos S， Cresci GAM. The gut barrier and chronic diseases[J]. Curr Opin Clin Nutr Metab Care，2022，25（3）：178-185.
本文引用 [1]
10
Vanuytsel T， Tack J， Farre R. The role of intestinal permeability in gastrointestinal disorders and current methods of evaluation[J]. Front Nutr，2021，8：717925.
本文引用 [1]
11
Odenwald MA， Turner JR. Intestinal permeability defects：is it time to treat?[J]. Clin Gastroenterol Hepatol，2013，11（9）：1075-1083.
本文引用 [1]
12
Bischoff SC， Barbara G， Buurman W，et al. Intestinal permeability：a new target for disease prevention and therapy[J]. BMC Gastroenterol，2014，14：189.
本文引用 [1]
13
Odenwald MA， Turner JR. The intestinal epithelial barrier：a therapeutic target?[J]. Nat Rev Gastroenterol Hepatol，2017，14（1）：9-21.
本文引用 [1]
14
Vancamelbeke M， Vermeire S. The intestinal barrier：a fundamental role in health and disease[J]. Expert Rev Gastroenterol Hepatol，2017，11（9）：821-834.
本文引用 [1]
15
Gilani S， Howarth GS， Kitessa SM，et al. New biomarkers for increased intestinal permeability induced by dextran sodium sulphate and fasting in chickens[J]. J Anim Physiol Anim Nutr，2017，101（5）：e237-e245.
本文引用 [1]
16
Liebe H， Schlegel C， Cai X，et al. Determining Intestinal Permeability using Lucifer Yellow in an Apical-Out Enteroid Model[J]. J Vis Exp，2022（185）：e64215.
本文引用 [1]
17
Soyseven M， Kaynak MS， Çelebier M，et al. Development of a RP-HPLC method for simultaneous determination of reference markers used for in situ rat intestinal permeability studies[J]. J Chromatogr B Analyt Technol Biomed Life Sci，2020，1147：122150.
本文引用 [1]
18
Hollander D， Kaunitz JD. The “leaky gut” ：tight junctions but loose associations?[J]. Dig Dis Sci，2020，65（5）：1277-1287.
本文引用 [1]
19
Munford RS. Endotoxemia-menace，marker，or mistake?[J]. J Leukoc Biol，2016，100（4）：687-698.
本文引用 [1]
20
Gnauck A， Lentle RG， Kruger MC. Chasing a ghost?：issues with the determination of circulating levels of endotoxin in human blood[J]. Crit Rev Clin Lab Sci，2016，53（3）：197-215.
本文引用 [1]
21
Vanslembrouck B， Chen JH， Larabell C，et al. Microscopic visualization of cell-cell adhesion complexes at micro and nanoscale[J]. Front Cell Dev Biol，2022，10：819534.
本文引用 [1]
22
Citronberg JS， Wilkens LR， Lim U，et al. Reliability of plasma lipopolysaccharide-binding protein（LBP） from repeated measures in healthy adults[J]. Cancer Causes Control，2016，27（9）：1163-1166.
本文引用 [1]
23
Vemuri R， Gundamaraju R， Eri R. Role of lactic acid probiotic bacteria in IBD[J]. Curr Pharm Des，2017，23（16）：2352-2355.
本文引用 [1]
24
Remund B， Yilmaz B， Sokollik C. D-lactate：implications for gastrointestinal diseases[J]. Children，2023，10（6）：945.
本文引用 [1]
25
Fukuda T， Tsukano K， Nakatsuji H，et al. Plasma diamine oxidase activity decline with diarrhea severity in calves indicating systemic dysfunction related to intestinal mucosal damage[J]. Res Vet Sci，2019，126：127-130.
本文引用 [1]
26
Cai JR， Chen H， Weng ML，et al. Diagnostic and clinical significance of serum levels of D-lactate and diamine oxidase in patients with Crohn’s disease[J]. Gastroenterol Res Pract，2019，2019：8536952.
本文引用 [1]
27
Izquierdo-Casas J， Comas-Basté O， Latorre-Moratalla ML，et al. Low serum diamine oxidase（DAO） activity levels in patients with migraine[J]. J Physiol Biochem，2018，74（1）：93-99.
本文引用 [1]
28
Takiishi T， Fenero CIM， Câmara NOS. Intestinal barrier and gut microbiota：shaping our immune responses throughout life[J]. Tissue Barriers，2017，5（4）：e1373208.
本文引用 [1]
29
Johansson ME， Gustafsson JK， Holmén-Larsson J，et al. Bacteria penetrate the normally impenetrable inner colon mucus layer in both murine colitis models and patients with ulcerative colitis[J]. Gut，2014，63（2）：281-291.
本文引用 [2]
30
Maric S， Flüchter P， Guglielmetti LC，et al. Plasma citrulline correlates with basolateral amino acid transporter LAT4 expression in human small intestine[J]. Clin Nutr，2021，40（4）：2244-2251.
本文引用 [1]
31
Curis E， Nicolis I， Moinard C，et al. Almost all about citrulline in mammals[J]. Amino Acids，2005，29（3）：177-205.
本文引用 [2]
32
Wells JM， Brummer RJ， Derrien M，et al. Homeostasis of the gut barrier and potential biomarkers[J]. Am J Physiol Gastrointest Liver Physiol，2017，312（3）：G171-G193.
本文引用 [1]
33
Derikx JP， Blijlevens NM， Donnelly JP，et al. Loss of enterocyte mass is accompanied by diminished turnover of enterocytes after myeloablative therapy in haematopoietic stem-cell transplant recipients[J]. Ann Oncol，2009，20（2）：337-342.
本文引用 [1]
34
Grootjans J， Thuijls G， Verdam F，et al. Non-invasive assessment of barrier integrity and function of the human gut[J]. World J Gastrointest Surg，2010，2（3）：61-69.
本文引用 [1]
35
Malcomson FC， Willis ND， McCallum I，et al. Effects of supplementation with nondigestible carbohydrates on fecal calprotectin and on epigenetic regulation of SFRP1 expression in the large-bowel mucosa of healthy individuals[J]. Am J Clin Nutr，2017，105（2）：400-410.
本文引用 [1]
36
D’Amico F， Nancey S， Danese S，et al. A practical guide for faecal calprotectin measurement：myths and realities[J]. J Crohns Colitis，2021，15（1）：152-161.
本文引用 [1]
37
Damms A， Bischoff SC. Validation and clinical significance of a new calprotectin rapid test for the diagnosis of gastrointestinal diseases[J]. Int J Colorectal Dis，2008，23（10）：985-992.
本文引用 [1]
38
Schwiertz A， Spiegel J， Dillmann U，et al. Fecal markers of intestinal inflammation and intestinal permeability are elevated in Parkinson’s disease[J]. Parkinsonism Relat Disord，2018，50：104-107.
本文引用 [1]
39
Kosek M， Haque R， Lima A，et al. Fecal markers of intestinal inflammation and permeability associated with the subsequent acquisition of linear growth deficits in infants[J]. Am J Trop Med Hyg，2013，88（2）：390-396.
本文引用 [1]
40
Ajamian M， Steer D， Rosella G，et al. Serum zonulin as a marker of intestinal mucosal barrier function：may not be what it seems[J]. PLoS One，2019，14（1）：e0210728.
本文引用 [1]
41
Zhang C， Zhu HP， Jie H，et al. Arbutin ameliorated ulcerative colitis of mice induced by dextran sodium sulfate（DSS）[J]. Bioengineered，2021，12（2）：11707-11715.
本文引用 [1]
42
Wang JL， Han X， Li JX，et al. Differential analysis of intestinal microbiota and metabolites in mice with dextran sulfate sodium-induced colitis[J]. World J Gastroenterol，2022，28（43）：6109-6130.
本文引用 [1]
43
Cabinian A， Sinsimer D， Tang M，et al. Gut symbiotic microbes imprint intestinal immune cells with the innate receptor SLAMF4 which contributes to gut immune protection against enteric pathogens[J]. Gut，2018，67（5）：847-859.
本文引用 [1]
44
Cortez V， Boyd DF， Crawford JC，et al. Astrovirus infects actively secreting goblet cells and alters the gut mucus barrier[J]. Nat Commun，2020，11（1）：2097.
45
Rusticeanu M， Zimmer V， Lammert F. Visualising and quantifying intestinal permeability-where do we stand[J]. Ann Hepatol，2021，23：100266.
本文引用 [1]
46
Chiriac S， Sfarti CV， Minea H，et al. Impaired intestinal permeability assessed by confocal laser endomicroscopy-a new potential therapeutic target in inflammatory bowel disease[J]. Diagnostics，2023，13（7）：1230.
本文引用 [4]
47
Ussing HH， Zerahn K. Active transport of sodium as the source of electric current in the short-circuited isolated frog skin[J]. Acta Physiol Scand，1951，23（2/3）：110-127.
本文引用 [1]
48
Biskou O， Jauregi-Miguel A. Measuring intestinal permeability in celiac disease ex vivo，using Ussing chambers[J]. Methods Cell Biol，2023，179：21-38.
本文引用 [1]
49
Galipeau HJ， Verdu EF. The complex task of measuring intestinal permeability in basic and clinical science[J]. Neurogastroenterol Motil，2016，28（7）：957-965.
本文引用 [2]
50
Roberts CL， Keita AV， Duncan SH，et al. Translocation of Crohn’s disease Escherichia coli across M-cells：contrasting effects of soluble plant fibres and emulsifiers[J]. Gut，2010，59（10）：1331-1339.
本文引用 [1]
51
Devriese S， van den Bossche L， van Welden S，et al. T84 monolayers are superior to Caco-2 as a model system of colonocytes[J]. Histochem Cell Biol，2017，148（1）：85-93.
本文引用 [1]
52
Chopyk DM， Kumar P， Raeman R，et al. Dysregulation of junctional adhesion molecule-a contributes to ethanol-induced barrier disruption in intestinal epithelial cell monolayers[J]. Physiol Rep，2017，5（23）：e13541.
本文引用 [1]
53
Naydenov NG， Brown B， Harris G，et al. A membrane fusion protein αSNAP is a novel regulator of epithelial apical junctions[J]. PLoS One，2012，7（4）：e34320.
本文引用 [1]
54
Sun L， Cao XL， Lechuga S，et al. A septin cytoskeleton-targeting small molecule，forchlorfenuron，inhibits epithelial migration via septin-independent perturbation of cellular signaling[J]. Cells，2019，9（1）：84.
本文引用 [1]
55
Wang DD， Naydenov NG， Feygin A，et al. Actin-depolymerizing factor and cofilin-1 have unique and overlapping functions in regulating intestinal epithelial junctions and mucosal inflammation[J]. Am J Pathol，2016，186（4）：844-858.
本文引用 [1]
56
Dosh RH， Jordan-Mahy N， Sammon C，et al. Tissue engineering laboratory models of the small intestine[J]. Tissue Eng Part B Rev，2018，24（2）：98-111.
本文引用 [1]
57
Rahmani S， Breyner NM， Su HM，et al. Intestinal organoids：a new paradigm for engineering intestinal epithelium in vitro [J]. Biomaterials，2019，194：195-214.
本文引用 [1]
58
林晓宇，李　琛，张婷，等. 肝硬化门静脉高压症患者肠壁屏障功能的改变及其与易发感染的关系[J]. 中华肝脏病杂志，2023，31（1）：70-76.
Lin XY， Li C， Zhang T，et al. Changes of intestinal wall barrier function and its correlation with susceptibility to infection in patients with cirrhotic portal hypertension[J]. Chin J Hepatol，2023，31（1）：70-76.
本文引用 [1]
59
Holthaus D， Kraft MR， Krug SM，et al. Dissection of barrier dysfunction in organoid-derived human intestinal epithelia induced by Giardia duodenalis[J]. Gastroenterology，2022，162（3）：844-858.
本文引用 [1]
60
Tse CM， In JG， Yin JY，et al. Enterohemorrhagic E. coli（EHEC）-secreted serine protease EspP stimulates electrogenic ion transport in human colonoid monolayers[J]. Toxins，2018，10（9）：351.
本文引用 [1]
61
Moorefield EC， Blue RE， Quinney NL，et al. Generation of renewable mouse intestinal epithelial cell monolayers and organoids for functional analyses[J]. BMC Cell Biol，2018，19（1）：15.
本文引用 [1]
62
van Dooremalen WTM， Derksen M， Roos JL，et al. Organoid-derived epithelial monolayer：a clinically relevant in vitro model for intestinal barrier function[J]. J Vis Exp，2021（173）：e6207.
本文引用 [1]
63
Santacroce G， Zammarchi I， Tan CK，et al. Present and future of endoscopy precision for inflammatory bowel disease[J]. Dig Endosc，2024，36（3）：292-304.
本文引用 [1]
64
Sturm A， Maaser C， Calabrese E，et al. ECCO-ESGAR Guideline for Diagnostic Assessment in IBD Part 2：IBD scores and general principles and technical aspects[J]. J Crohns Colitis，2019，13（3）：273-284.
本文引用 [1]
65
Michiba K， Watanabe K， Imaoka T，et al. Recent advances in the gastrointestinal complex in vitro model for ADME studies[J]. Pharmaceutics，2023，16（1）：37.
本文引用 [1]

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