Objective To assess the effect of different tubal treatments on ovarian reserve status and assisted reproductive technology （ART） outcomes in infertile patients. Methods This study included 11316 patients receiving ART in Chongqing Institute of Reproduction and Genetics，Chongqing Health Center for Women and Children from January 2017 to September 2022. The patients were divided into three groups： non-surgery group（n=4 732），conservative tubal surgery group（n=4 578），and tubal ligation/salpingectomy group（n=2 006）. The primary outcome measures were serum anti-mullerian hormone（AMH），basic antral follicle count（AFC），basic follicle-stimulating hormone（FSH），number of oocytes retrieved，and number of transferable embryos. One-way analysis of variance，chi-square test，and generalized linear model （least significant difference） were used for analysis. Results Compared with the non-surgery group，the patient’ ovarian reserve function decreased significantly in the conservative tubal surgery group，with significant differences in AMH（OR=0.736，95%CI=0.668-0.811，P=0.001），basic FSH（OR=1.171，95%CI=1.064-1.288，P=0.001），AFC（OR=0.600，95%CI=0.519-0.693，P=0.001），number of oocytes retrieved（OR=0.569，95%CI=0.439-0.739，P=0.001），and number of transferable embryos（OR=0.788，95%CI=0.703-0.883，P<0.001）；the ovarian reserve function also decreased significantly in the tubal ligation/salpingectomy group，with significant differences in AMH（OR=0.752，95%CI=0.660-0.856，P<0.001），basic FSH （OR=1.160，95%CI=1.021-1.318，P<0.05），and AFC（OR=0.549，95%CI=0.453-0.665，P<0.001）. No significant differences were observed in AMH，basic FSH，AFC，and number of oocytes retrieved between the tubal ligation/salpingectomy group and the conservative tubal surgery group，but there were positive effects on the number of metaphase Ⅱ oocytes（OR=1.564，95%CI=1.129-2.167，P=0.007） and number of transferable embryos（OR=1.270，95%CI=1.091-1.479，P=0.002）. Conclusion The ovarian reserve function decreases in infertile women after tubal ligation/salpingectomy or conservative tubal surgery. However，tubal ligation/salpingectomy do not result in more serious ovarian reserve impairment compared with conservative tubal surgery.