Codonopsis pilosula polysaccharide inhibits the proliferation of keloid fibroblasts and induces cell cycle arrest through PI3K/AKT signaling pathway: an experimental study

Tian Yan, Zhu Fei, Song Caiying, Zhu Qiuxuan, Cheng Shengrong, Chen Wendong

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Journal of Chongqing Medical University ›› 2024, Vol. 49 ›› Issue (09) : 1142-1146. DOI: 10.13406/j.cnki.cyxb.003580
Basic research

Codonopsis pilosula polysaccharide inhibits the proliferation of keloid fibroblasts and induces cell cycle arrest through PI3K/AKT signaling pathway: an experimental study

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Abstract

Objective To investigate the effects of Codonopsis pilosula polysaccharide on the proliferation and cell cycle progression of human keloid fibroblasts，and the possible molecular mechanisms. Methods The 3-（4，5-Dimethylthazol-2-yl）-2，5-diphenyltetrazolium bromide assay was used to determine the effect of C. pilosula polysaccharide on cell survival rate and select the concentration of C. pilosula polysaccharide. The clone-forming ability of keloid fibroblasts was determined by plate cloning experiment. The cell cycle distribution and apoptosis were determined by flow cytometry. Western blot was used to analyze proliferating cell nuclear antigen，cyclin D1，collagen I，collagen Ⅲ，and the phosphorylation levels of phosphatidylinositol 3-kinase and protein kinase B. Results When the concentration of C. pilosula polysaccharide was less than 80 μmol/L，there was no obvious toxic effect on keloid fibroblasts. In this experiment，C. pilosula polysaccharide was added at the concentrations of 10，20，and 40 μmol/L. Compared with the 0 μmol/L group，the clone-forming ability of keloid fibroblasts decreased in the 10，20，and 40 μmol/L groups； cell cycle was arrested at the G0/G1 phase； the expression of proliferating cell nuclear antigen，cyclin D1，collagen Ⅰ，and collagen Ⅲ was down-regulated； cell apoptosis was increased； the phosphorylation levels of PI3K and AKT decreased （P<0.05）. Conclusion Codonopsispilosula polysaccharide can inhibit the proliferation and cell cycle progression of keloid fibroblasts，and the molecular mechanisms may be related to the inhibition of the PI3K/AKT signal pathway.

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Codonopsis pilosula polysaccharide / keloid fibroblasts / proliferation / cell cycle / PI3K/AKT signaling pathway

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Tian Yan , Zhu Fei , Song Caiying , et al . Codonopsis pilosula polysaccharide inhibits the proliferation of keloid fibroblasts and induces cell cycle arrest through PI3K/AKT signaling pathway: an experimental study. Journal of Chongqing Medical University. 2024, 49(09): 1142-1146 https://doi.org/10.13406/j.cnki.cyxb.003580

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