An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells

Cao Yuchao, Liao Hongjian, Hu Can, Guo Jiajun, Du Yonghong

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Journal of Chongqing Medical University ›› 2024, Vol. 49 ›› Issue (09) : 1105-1112. DOI: 10.13406/j.cnki.cyxb.003578
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An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells

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Abstract

Objective To investigate the effects on the proliferation，migration and invasion of Lewis lung carcinoma（LLC） cells induced by DNA nanoflowers（DFs） carrying doxorubicin（DOX） and hypoxia inducible factor-1α（HIF-1α） antisense oligonucleotides. Methods DFs containing HIF-1α antisense oligonucleotides（DHA-DF） were synthesized by rolling circle amplification，and the physical properties of DHA-DF were examined. DOX was loaded into DFs to form DDF.We evaluated the cell viability of LLC cells treated with DHA-DDF by using Cell Counting Kit-8； assessed the effects of DHA-DDF on the migration and invasion of LLC cells by using scratch assay and Transwell assay； and measured the expression level of HIF-1α in LLC cells by Western blot and quantitative polymerase chain reaction. Results DHA-DF appeared flower-like porous spherical particles with a size of （421.26±7.90） nm. Compare with free DOX and DA-DDF，DHA-DDF significantly decreased the mRNA and protein expression of HIF-1α in LLC cells，and significantly reduced cell viability，the area of wound healed and the number of invasive cells（all P<0.05）. Conclusion DHA-DDF could effectively reduce the expression of HIF-1α，and inhibit the proliferation，migration and invasion of LLC cells，providing new ideas for clinical treatment of tumors.

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DNA nanoflower / hypoxia / lung cancer / antisense oligonucleotide

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Cao Yuchao , Liao Hongjian , Hu Can , et al . An experimental study of editable DNA nanoflowers inhibiting the proliferation，migration and invasion of Lewis lung carcinoma cells. Journal of Chongqing Medical University. 2024, 49(09): 1105-1112 https://doi.org/10.13406/j.cnki.cyxb.003578

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
1
Huang XL， Zhuang J， Chung SW，et al. Hypoxia-tropic protein nanocages for modulation of tumor- and chemotherapy-associated hypoxia[J]. ACS Nano，2019，13（1）：236-247．
本文引用 [1]
2
Li ML， Xia J， Tian RS，et al. Near-infrared light-initiated molecular superoxide radical generator：rejuvenating photodynamic therapy against hypoxic tumors[J]. J Am Chem Soc，2018，140（44）：14851-14859．
3
Chen J， Luo HL， Liu Y，et al. Oxygen-self-produced nanoplatform for relieving hypoxia and breaking resistance to sonodynamic treatment of pancreatic cancer[J]. ACS Nano，2017，11（12）：12849-12862．
本文引用 [1]
4
Park JH， Moon M， Kim JS，et al. TOPK mediates hypoxia-induced epithelial-mesenchymal transition and the invasion of nonsmall-cell lung cancer cells via the HIF-1α/snail axis[J]. Biochem Biophys Res Commun，2021，534：941-949．
本文引用 [1]
5
Vito A， El-Sayes N， Mossman K. Hypoxia-driven immune escape in the tumor microenvironment[J]. Cells，2020，9（4）：992．
本文引用 [1]
6
Wu QH， You L， Nepovimova E，et al. Hypoxia-inducible factors：master regulators of hypoxic tumor immune escape[J]. J Hematol Oncol，2022，15（1）：77．
本文引用 [1]
7
Wang K， Zhu X， Zhang K，et al. Interleukin-6 contributes to chemoresistance in MDA-MB-231 cells via targeting HIF-1α[J]. J Biochem Mol Toxicol，2018，32（3）：e22039．
本文引用 [1]
8
Li H， Quan JS， Zhang MZ，et al. Lipid-albumin nanoparticles（LAN） for therapeutic delivery of antisense oligonucleotide against HIF-1α[J]. Mol Pharm，2016，13（7）：2555-2562．
本文引用 [1]
9
Pan M， Jiang QY， Sun JL，et al. Programming DNA nanoassembly for enhanced photodynamic therapy[J]. Angew Chem Int Ed，2020，59（5）：1897-1905．
本文引用 [3]
10
Crooke ST， Baker BF， Crooke RM，et al. Antisense technology：an overview and prospectus[J]. Nat Rev Drug Discov，2021，20（6）：427-453．
本文引用 [1]
11
Bennett CF， Baker BF， Pham N，et al. Pharmacology of antisense drugs[J]. Annu Rev Pharmacol Toxicol，2017，57：81-105．
本文引用 [1]
12
Mei L， Zhu GZ， Qiu LP，et al. Self-assembled multifunctional DNA nanoflowers for the circumvention of multidrug resistance in targeted anticancer drug delivery[J]. Nano Res，2015，8（11）：3447-3460．
本文引用 [2]
13
Kuerban K， Gao XW， Zhang H，et al. Doxorubicin-loaded bacterial outer-membrane vesicles exert enhanced anti-tumor efficacy in non-small-cell lung cancer[J]. Acta Pharm Sin B，2020，10（8）：1534-1548．
本文引用 [1]
14
Dai ZD， Song XZ， Cao JK，et al. Dual-stimuli-responsive TiO x /DOX nanodrug system for lung cancer synergistic therapy[J]. RSC Adv，2018，8（39）：21975-21984．
本文引用 [1]
15
Bhatia SN， Chen XY， Dobrovolskaia MA，et al. Cancer nanomedicine[J]. Nat Rev Cancer，2022，22（10）：550-556．
本文引用 [1]
16
Rizvi SAA， Saleh AM. Applications of nanoparticle systems in drug delivery technology[J]. Saudi Pharm J，2018，26（1）：64-70．
本文引用 [1]
17
Bie LH， Wang Y， Jiang FZ，et al. Insights into the binding mode of AS1411 aptamer to nucleolin[J]. Front Mol Biosci，2022，9：1025313．
本文引用 [1]
18
Gao YT， Zhou H， Liu GG，et al. Tumor microenvironment：lactic acid promotes tumor development[J]. J Immunol Res，2022，2022：3119375．
本文引用 [1]
19
Keizer HG， Pinedo HM， Schuurhuis GJ，et al. Doxorubicin（adriamycin）：a critical review of free radical-dependent mechanisms of cytotoxicity[J]. Pharmacol Ther，1990，47（2）：219-231．
本文引用 [1]
20
He YX， Liu SH， Yin J，et al. Sonodynamic and chemodynamic therapy based on organic/organometallic sensitizers[J]. Coord Chem Rev，2021，429：213610．
本文引用 [2]
21
Cheng JJ， Zhu Y， Xing X，et al. Manganese-deposited iron oxide promotes tumor-responsive ferroptosis that synergizes the apoptosis of cisplatin[J]. Theranostics，2021，11（11）：5418-5429．
本文引用 [1]
22
Li L， Chen YS， Chen WJ，et al. Photodynamic therapy based on organic small molecular fluorescent dyes[J]. Chin Chem Lett，2019，30（10）：1689-1703．
本文引用 [1]

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