Has-miR-204-5p mediates intervertebral disc degeneration and associated pain symptoms: evidence from Mendelian randomization and bioinformatics

Li Ningdao, Zhao Runhan, Zhang Jun, Tang Dagang, Luo Xiaoji

PDF(3835 KB)
PDF(3835 KB)
Journal of Chongqing Medical University ›› 2024, Vol. 49 ›› Issue (08) : 1033-1038. DOI: 10.13406/j.cnki.cyxb.003554
Bioinformatics

Has-miR-204-5p mediates intervertebral disc degeneration and associated pain symptoms: evidence from Mendelian randomization and bioinformatics

Author information +
History +

Abstract

Objective To identify novel MicroRNA biomarkers of intervertebral disc degeneration（IDD） and related pain symptoms，and to explore the underlying molecular mechanisms. Methods Genome-wide association studies（GWAS） data for MicroRNAs，back pain，and sciatica were obtained from a published article and the GWAS Catlog database. IDD-associated MicroRNA and mRNA expression data were downloaded from the GEO database. Key MicroRNAs were identified using Mendelian randomization and bioinformatics analyses. The biological functions of their target genes were explored based on the GeneMANIA database. Results Mendelian randomization analysis showed that has-miR-204-5p had significant causal effects with both back pain（GCST90018797，OR=0.9761） and sciatica（GCST90044614，OR=0.8957）. Moreover，bioinformatics analysis revealed that has-miR-204-5p was significantly less expressed in IDD tissues，and its three target genes（COL3A1，RPLP1，and TCF4） were abnormally highly expressed in IDD tissues. In addition，biological function enrichment analysis revealed that the three target genes were mainly enriched in biological functions closely related to IDD，such as collagen，ribosome，and the Wnt signaling pathway. Conclusion Has-miR-204-5p is a reliable IDD biomarker that plays an important role in IDD and associated pain symptoms.

Key words

intervertebral disc degeneration / Mendelian randomization / bioinformatics / has-miR-204-5p

Cite this article

EndNote

Ris (Procite)

Bibtex

Download Citations
Li Ningdao , Zhao Runhan , Zhang Jun , et al . Has-miR-204-5p mediates intervertebral disc degeneration and associated pain symptoms: evidence from Mendelian randomization and bioinformatics. Journal of Chongqing Medical University. 2024, 49(08): 1033-1038 https://doi.org/10.13406/j.cnki.cyxb.003554

References

List( Publishing order | Descend order by publishing year | Descend order by cited within ) Chart analysis
1
Katz JN. Lumbar disc disorders and low-back pain：socioeconomic factors and consequences[J]. J Bone Joint Surg Am，2006，88 Suppl 2：S21-S24．
本文引用 [1]
2
Yang ZD， Chen X， Zhang QL，et al. Dysregulated COL3A1 and RPL8，RPS16，and RPS23 in disc degeneration revealed by bioinformatics methods[J]. Spine，2015，40（13）：745-751．
本文引用 [2]
3
Fritz JM， Lane E， McFadden M，et al. Physical therapy referral from primary care for acute back pain with sciatica：a randomized controlled trial[J]. Ann Intern Med，2021，174（1）：8-17．
本文引用 [1]
4
Konstantinou K， Dunn KM. Sciatica：review of epidemiological studies and prevalence estimates[J]. Spine，2008，33（22）：2464-2472．
5
Hurwitz EL， Randhawa K， Yu HN，et al. The Global Spine Care Initiative：a summary of the global burden of low back and neck pain studies[J]. Eur Spine J，2018，27 Suppl 6：S796-S801.
本文引用 [1]
6
Vroomen PCAJ， de Krom MCTFM， Knottnerus JA. Predicting the outcome of sciatica at short-term follow-up[J]. Br J Gen Pract，2002，52（475）：119-123．
本文引用 [1]
7
Konstantinou K， Dunn KM， Ogollah R，et al. Prognosis of sciatica and back-related leg pain in primary care：the ATLAS cohort[J]. Spine J，2018，18（6）：1030-1040．
本文引用 [1]
8
Mehling WE， Gopisetty V， Bartmess E，et al. The prognosis of acute low back pain in primary care in the United States：a 2-year prospective cohort study[J]. Spine，2012，37（8）：678-684．
本文引用 [1]
9
Grimson A， Farh KK， Johnston WK，et al. MicroRNA targeting specificity in mammals：determinants beyond seed pairing[J]. Mol Cell，2007，27（1）：91-105．
本文引用 [1]
10
Ho PTB， Clark IM， Le LTT. MicroRNA-based diagnosis and therapy[J]. Int J Mol Sci，2022，23（13）：7167．
本文引用 [1]
11
Li Z， Yu X， Shen JX，et al. MicroRNA in intervertebral disc degeneration[J]. Cell Prolif，2015，48（3）：278-283．
本文引用 [1]
12
Liu HZ， Huang XW， Liu XY，et al. miR-21 promotes human nucleus pulposus cell proliferation through PTEN/AKT signaling[J]. Int J Mol Sci，2014，15（3）：4007-4018．
本文引用 [1]
13
Wang HQ， Yu XD， Liu ZH，et al. Deregulated miR-155 promotes Fas-mediated apoptosis in human intervertebral disc degeneration by targeting FADD and caspase-3[J]. J Pathol，2011，225（2）：232-242．
本文引用 [1]
14
Tsirimonaki E， Fedonidis C， Pneumaticos SG，et al. PKCε signalling activates ERK1/2，and regulates aggrecan，ADAMTS5，and miR377 gene expression in human nucleus pulposus cells[J]. PLoS One，2013，8（11）：e82045．
本文引用 [1]
15
Bowden J， Holmes MV. Meta-analysis and Mendelian randomization：a review[J]. Res Synth Methods，2019，10（4）：486-496．
本文引用 [1]
16
Zuccolo L， Holmes MV. Commentary：Mendelian randomization-inspired causal inference in the absence of genetic data[J]. Int J Epidemiol，2017，46（3）：962-965．
本文引用 [1]
17
Huan TX， Rong J， Liu CY，et al. Genome-wide identification of microRNA expression quantitative trait loci[J]. Nat Commun，2015，6：6601．
本文引用 [1]
18
Yoshioka K， Takemura T， Tohda M，et al. Glomerular localization of type Ⅲ collagen in human kidney disease[J]. Kidney Int，1989，35（5）：1203-1211．
本文引用 [1]
19
Legrand A， Guery C， Faugeroux J，et al. Comparative therapeutic strategies for preventing aortic rupture in a mouse model of vascular Ehlers-Danlos syndrome[J]. PLoS Genet，2022，18（3）：e1010059．
20
Watanabe A， Shimada T. Vascular type of Ehlers-Danlos syndrome[J]. J Nippon Med Sch，2008，75（5）：254-261．
21
Ucaryilmaz Metin C， Ozcan G. Comprehensive bioinformatic analysis reveals a cancer-associated fibroblast gene signature as a poor prognostic factor and potential therapeutic target in gastric cancer[J]. BMC Cancer，2022，22（1）：692．
本文引用 [1]
22
Gruber HE， Hoelscher GL， Ingram JA，et al. Variations in aggrecan localization and gene expression patterns characterize increasing stages of human intervertebral disk degeneration[J]. Exp Mol Pathol，2011，91（2）：534-539．
本文引用 [1]
23
Cevei M， Roşca E， Liviu L，et al. Imagistic and histopathologic concordances in degenerative lesions of intervertebral disks[J]. Rom J Morphol Embryol，2011，52（1 Suppl）：327-332．
本文引用 [1]
24
Ordaz A， Anderson B， Zlomislic V，et al. Paraspinal muscle gene expression across different aetiologies in individuals undergoing surgery for lumbar spine pathology[J]. Eur Spine J，2023，32（4）：1123-1131．
本文引用 [1]
25
Chen WH， Lo WC， Lee JJ，et al. Tissue-engineered intervertebral disc and chondrogenesis using human nucleus pulposus regulated through TGF-beta1 in platelet-rich plasma[J]. J Cell Physiol，2006，209（3）：744-754．
本文引用 [1]
26
Perucho L， Artero-Castro A， Guerrero S，et al. RPLP1，a crucial ribosomal protein for embryonic development of the nervous system[J]. PLoS One，2014，9（6）：e99956．
本文引用 [1]
27
He ZX， Xu Q， Wang X，et al. RPLP1 promotes tumor metastasis and is associated with a poor prognosis in triple-negative breast cancer patients[J]. Cancer Cell Int，2018，18：170．
本文引用 [1]
28
Forrest MP， Hill MJ， Quantock AJ，et al. The emerging roles of TCF4 in disease and development[J]. Trends Mol Med，2014，20（6）：322-331．
本文引用 [1]
29
Papes F， Camargo AP， de Souza JS，et al. Transcription Factor 4 loss-of-function is associated with deficits in progenitor proliferation and cortical neuron content[J]. Nat Commun，2022，13（1）：2387．
本文引用 [1]
30
范东东，原向伟. Wnt信号通路在椎间盘退变中的作用机制研究进展[J]. 医药前沿，2023，9（6）：67-70．
Fan DD， Yuan XW. Research progress on the mechanism of Wnt signaling pathway in intervertebral disc degeneration[J]. J Front Med，2023，9（6）：67-70．
本文引用 [1]

Comments

PDF(3835 KB)

Accesses

Citation

Detail
Sections
Recommended
Copyright © Journal of Chongqing Medical University, All Rights Reserved.
Powered by Beijing Magtech Co. Ltd.

/

〈 〉