Effects of different intensities of aerobic exercise on the kidney of spontaneously hypertensive rats

He Longlin, Cao Shuyuan, Wang Yang, Chang Qing

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Journal of Chongqing Medical University ›› 2024, Vol. 49 ›› Issue (07) : 830-837. DOI: 10.13406/j.cnki.cyxb.003546
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Effects of different intensities of aerobic exercise on the kidney of spontaneously hypertensive rats

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Abstract

Objective To study the effects of aerobic exercise on the expression of pyroptosis-related proteins in the kidney of spontaneously hypertensive rats（SHRs），and to explore the effects of aerobic exercise for hypertensive renal damage in SHRs. Methods Eight-week-old male SHRs and Wistar Kyoto rats（WKYs） were randomly divided into four groups（n=6 in each group）： control group（WKY），non-exercise hypertension group（SHR-N），low-intensity aerobic exercise hypertension group（SHR-L），and moderate-intensity aerobic exercise hypertension group（SHR-M）. The rats in the SHR-L and SHR-M groups were subjected to running on a sports treadmill at a slope of 0° at 12-14 m/min and 18-20 m/min for 60 min，respectively，for 5 times per week. The WKY and SHR-N groups had no exercise intervention. After 10 weeks，72 hours after exercise，we measured the blood pressure of rats；measured serum creatinine（Cr），urea nitrogen（BUN），interleukin（IL）-1β，and IL-18；observed the pathological changes in the kidney with Masson staining，and calculated the renal function index（RFI） to reflect the severity of renal function impairment；and measured the protein expression of nucleotide-binding oligomerization domain-like receptor protein 3（NLRP3），cysteinyl aspartate-specific proteinase 1（caspase-1），cleaved caspase-1，toll-like receptor 4（TLR4），IL-1β，IL-18，gasdermin D（GSDMD），and GSDMDC1 in the renal tissue of rats. Results Compared with the WKY group，the SHR-N group showed significant increases in blood pressure，serum Cr，BUN，IL-1β，and IL-18 levels，and RFI，as well as the expression of NLRP3，caspase-1，TLR4，IL-1β，IL-18，GSDMD，and GSDMDC1 in the kidney. Compared with the SHR-N group，the SHR-M group showed significantly decreased blood pressure，serum Cr and BUN，and RFI，as well as NLRP3，caspase-1，TLR4，IL-1β，IL-18，GSDMD，and GSDMDC1，while the SHR-L group had no significant reductions. Conclusion Different intensities of aerobic exercise produce varying degrees of effects on renal NLRP3 inflammasome expression，blood pressure，and renal function in SHRs. Moderate-intensity aerobic exercise is superior to low-intensity aerobic exercise in improving renal function in SHRs through the TLR4/NLRP3 signaling pathway.

Key words

nucleotide-binding oligomerization domain-like receptor protein 3 / spontaneously hypertensive rat / kidney / aerobic exercise / exercise intensity

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He Longlin , Cao Shuyuan , Wang Yang , et al. Effects of different intensities of aerobic exercise on the kidney of spontaneously hypertensive rats. Journal of Chongqing Medical University. 2024, 49(07): 830-837 https://doi.org/10.13406/j.cnki.cyxb.003546

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