Objective To explore the value of placental growth factor（PLGF），soluble fms-like tyrosine kinase-1（SFLT-1），and glycosylated fibronectin（GLYFN） in predicting preeclampsia. Methods A total of 188 pregnant women who visited Wuxi Maternal and Child Health Hospital were enrolled in the study and divided into control group（154 normal pregnant women） and preeclampsia group（34 patients with preeclampsia）. The concentrations of serum PLGF，SFLT-1，and GLYFN at the 16th to 18th weeks of gestation were measured by immunofluorescence assay. The levels of individual markers were compared between the preeclampsia group and the control group. The receiver operating characteristic（ROC） curves were used to assess the efficiency of the three markers in terms of predictive value. Results During the mid-pregnancy period，the preeclampsia group had a significantly lower concentration of serum PLGF but significantly higher concentrations of serum SFLT-1 and GLYFN compared with the control group（all P=0.000）. The areas under the ROC curve（AUC） for the 95% confidence interval were 0.941（0.907-0.974），0.881（0.800-0.962），and 0.951（0.918-0.985） for PLGF，SFLT-1，and GLYFN，respectively. The AUCs for SFLT-1 combined with GLYFN and the combined detection of the three indices were 0.968 and 0.986，respectively. Conclusion There are significant differences between the control group and the preeclampsia group in the marker levels of serum PLGF，SFLT-1，and GLYFN，which has some value in predicting the onset of preeclampsia. The predictive value of SFLT-1 combined with PLGF，SFLT-1 combined with GLYFN，and the combination of the three markers are greater than that of any single index.