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Gene Cloning, Expression and Interaction Protein Analysis of FERONIA in Brassica oleracea var. acephala
Baoru XUN, Hongtao QIN, Rui MA, Nanfeng GUO, Yunping LIU, Ying WU, Xingguo LAN
PDF(4370 KB)
PDF(4370 KB)
Gene Cloning, Expression and Interaction Protein Analysis of FERONIA in Brassica oleracea var. acephala
)FERONIA （FER） receptor kinase plays an important role in pollen and stigma recognition in flowering plants. To analyze the function of receptor-like kinase FERONIA（FER） in the pollination in ornamental kale（Brassica oleracea var. acephala）， BoFER gene was cloned from the stigma of the self-incompatibility line（S13-bS13-b ） of ornamental kale by RT-PCR， and the cloned cDNA sequence was 2 682 bp， which encoded 893 amino acids containing the highly conserved kinase domain and Ser/Thr kinase binding site， and the expression level of BoFER was analyzed by qRT-PCR. The results showed that the expression of BoFER in stigmas gradually increased after incompatible pollination， but gradually decreased after compatible pollination. Furthermore， the interaction between BoFER and known proteins related to self-incompatibility were analyzed by yeast two-hybrid. The results suggested that BoFER interacted with the kinase domain of S-locus receptor kinase BoSRK13-b.
Brassica oleracea var. acephala / FERONIA / self-incompatibility / expression analysis / yeast two-hybrid
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| 1 | CHEUNG A Y， DUAN Q H， LI C，et al.Pollen-pistil interactions：It takes two to tangle but a molecular cast of many to deliver［J］.Current Opinion in Plant Biology，2022，69：102279. |
| 2 | JOHNSON M A， HARPER J F， PALANIVELU R.A fruitful journey：pollen tube navigation from germination to fertilization［J］.Annual Review of Plant Biology，2019，70：809-837. |
| 3 | ABHINANDAN K， SANKARANARAYANAN S， MACGREGOR S，et al.Cell-cell signaling during the Brassicaceae self-incompatibility response［J］.Trends in Plant Science，2022，27（5）：472-487. |
| 4 | SWANSON R， EDLUND A F， PREUSS D.Species specificity in pollen-pistil interactions［J］.Annual Review of Genetics，2004，38：793-818. |
| 5 | DOUCET J， LEE H K， GORING D R.Pollen acceptance or rejection：a tale of two pathways［J］.Trends in Plant Science，2016，21（12）：1058-1067. |
| 6 | ZHANG L L， HUANG J B， SU S Q，et al.FERONIA receptor kinase-regulated reactive oxygen species mediate self-incompatibility in Brassica rapa ［J］.Current Biology，2021，31（14）：3004-3016.e4. |
| 7 | JANY E， NELLES H， GORING D R.The molecular and cellular regulation of Brassicaceae self-incompatibility and self-pollen rejection［J］.International Review of Cell and Molecular Biology，2019，343：1-35. |
| 8 | NASRALLAH J B.Self-incompatibility in the Brassicaceae：Regulation and mechanism of self-recognition［J］.Current Topics in Developmental Biology，2019，131：435-452. |
| 9 | NASRALLAH J B.Evolution of the Brassica self-incompatibility locus：a look into S-locus gene polymorphisms［J］.Proceedings of the National Academy of Sciences of the United States of America，1997，94（18）：9516-9519. |
| 10 | TAKAYAMA S， ISOGAI A.Self-incompatibility in plants［J］.Annual Review of Plant Biology，2005，56：467-489. |
| 11 | TAKAYAMA S， SHIMOSATO H， SHIBA H，et al.Direct ligand-receptor complex interaction controls Brassica self-incompatibility［J］.Nature，2001，413（6855）：534-538. |
| 12 | MURASE K， SHIBA H， IWANO M，et al.A membrane-anchored protein kinase involved in Brassica self-incompatibility signaling［J］.Science，2004，303（5663）：1516-1519. |
| 13 | KITASHIBA H， LIU P， NISHIO T，et al.Functional test of Brassica self-incompatibility modifiers in Arabidopsis thaliana ［J］.Proceedings of the National Academy of Sciences of the United States of America，2011，108（44）：18173-18178. |
| 14 | GU T S， MAZZURCO M， SULAMAN W，et al.Binding of an arm repeat protein to the kinase domain of the S-locus receptor kinase［J］.Proceedings of the National Academy of Sciences of the United States of America，1998，95（1）：382-387. |
| 15 | SAMUEL M A， CHONG Y T， HAASEN K E，et al.Cellular pathways regulating responses to compatible and self-incompatible pollen in Brassica and Arabidopsis stigmas intersect at Exo70A1，a putative component of the exocyst complex［J］.The Plant Cell，2009，21（9）：2655-2671. |
| 16 | SANKARANARAYANAN S， JAMSHED M， SAMUEL M A.Degradation of glyoxalase I in Brassica napus stigma leads to self-incompatibility response［J］.Nature Plants，2015，1：15185. |
| 17 | SCANDOLA S， SAMUEL M A.A flower-specific phospholipase D is a stigmatic compatibility factor targeted by the self-incompatibility response in Brassica napus ［J］.Current Biology，2019，29（3）：506-512.e4. |
| 18 | LI H J， YANG W C.RLKs orchestrate the signaling in plant male-female interaction［J］.Science China Life Sciences，2016，59（9）：867-877. |
| 19 | ZHONG S， QU L J.Peptide/receptor-like kinase-mediated signaling involved in male-female interactions［J］.Current Opinion in Plant Biology，2019，51：7-14. |
| 20 | YU Y Q， CHAKRAVORTY D， ASSMANN S M.The G protein β-subunit，AGB1，interacts with FERONIA in RALF1-regulated stomatal movement［J］.Plant Physiology，2018，176（3）：2426-2440. |
| 21 | FENG W， KITA D， PEAUCELLE A，et al.The FERONIA receptor kinase maintains cell-wall integrity during salt stress through Ca2+ signaling［J］.Current Biology，2018，28（5）：666-675.e5. |
| 22 | CHEN J， YU F， LIU Y，et al.FERONIA interacts with ABI2-type phosphatases to facilitate signaling cross-talk between abscisic acid and RALF peptide in Arabidopsis ［J］.Proceedings of the National Academy of Sciences of the United States of America，2016，113（37）：E5519-E5527. |
| 23 | YU F， QIAN L C， NIBAU C，et al.FERONIA receptor kinase pathway suppresses abscisic acid signaling in Arabidopsis by activating ABI2 phosphatase［J］.Proceedings of the National Academy of Sciences of the United States of America，2012，109（36）：14693-14698. |
| 24 | DUAN Q H， KITA D， LI C，et al.FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development［J］.Proceedings of the National Academy of Sciences of the United States of America，2010，107（41）：17821-17826. |
| 25 | DESLAURIERS S D， LARSEN P B.FERONIA is a key modulator of brassinosteroid and ethylene responsiveness in Arabidopsis hypocotyls［J］.Molecular Plant，2010，3（3）：626-640. |
| 26 | ESCOBAR-RESTREPO J M， HUCK N， KESSLER S，et al.The FERONIA receptor-like kinase mediates male-female interactions during pollen tube reception［J］.Science，2007，317（5838）：656-660. |
| 27 | GUO H Q， NOLAN T M， SONG G Y，et al.FERONIA receptor kinase contributes to plant immunity by suppressing jasmonic acid signaling in Arabidopsis thaliana ［J］.Current Biology，2018，28（20）：3316-3324.e6. |
| 28 | LI C， WU H M， CHEUNG A Y.FERONIA and her pals：functions and mechanisms［J］.Plant Physiology，2016，171（4）：2379-2392. |
| 29 | ROTMAN N， ROZIER F， BOAVIDA L，et al.Female control of male gamete delivery during fertilization in Arabidopsis thaliana ［J］.Current Biology，2003，13（5）：432-436. |
| 30 | HUCK N， MOORE J M， FEDERER M，et al.The Arabidopsis mutant feronia disrupts the female gametophytic control of pollen tube reception［J］.Development，2003，130（10）：2149-2159. |
| 31 | LINDNER H， MüLLER L M， BOISSON-DERNIER A，et al.CrRLK1L receptor-like kinases：not just another brick in the wall［J］.Current Opinion in Plant Biology，2012，15（6）：659-669. |
| 32 | TUNG C W， DWYER K G， NASRALLAH M E，et al.Genome-wide identification of genes expressed in Arabidopsis pistils specifically along the path of pollen tube growth［J］.Plant Physiology，2005，138（2）：977-989. |
| 33 | LIU C， SHEN L P， XIAO Y，et al.Pollen PCP-B peptides unlock a stigma peptide-receptor kinase gating mechanism for pollination［J］.Science，2021，372（6538）：171-175. |
| 34 | DUAN Q H， KITA D， JOHNSON E A，et al.Reactive oxygen species mediate pollen tube rupture to release sperm for fertilization in Arabidopsis ［J］.Nature Communications，2014，5（1）：3129. |
| 35 | DUAN Q H， LIU M C J， KITA D，et al.FERONIA controls pectin-and nitric oxide-mediated male-female interaction［J］.Nature，2020，579（7800）：561-566. |
| 36 | FRANKLIN-TONG N， BOSCH M.Plant biology：stigmatic ROS decide whether pollen is accepted or rejected［J］.Current Biology，2021，31（14）：R904-R906. |
| 37 | HUANG J B， YANG L， YANG L，et al.Stigma receptors control intraspecies and interspecies barriers in Brassicaceae［J］.Nature，2023，614（7947）：303-308. |
| 38 | 李阳，施亚坤，高士科，等.羽衣甘蓝类蛋白质二硫键异构酶PDIL1-2的基因克隆及蛋白表达分析［J］.植物研究，2020，40（2）：293-300. |
| 38 | LI Y， SHI Y K， GAO S K，et al.Gene cloning and expression analysis of PDIL1-2 of ornamental kale （Brassica oleracea var.acephala）［J］.Bulletin of Botanical Research，2020，40（2）：293-300. |
| 39 | 栗赫铭，秦洪涛，魏德强，等.羽衣甘蓝BoMAPK4原核表达、抗体制备及蛋白表达分析［J］.植物研究，2022，42（4）：584-591. |
| 39 | LI H M， QIN H T， WEI D Q，et al.Prokaryotic expression，preparation polyclonal antibody and protein expression of BoMAPK4 of ornamental kale （Brassica oleracea var.acephala）［J］.Bulletin of Botanical Research，2022，42（4）：584-591. |
| 40 | 李晗，李治龙，李晓屿，等.羽衣甘蓝不同组织及柱头发育实时荧光定量PCR内参基因的筛选［J］.植物研究，2016，36（4）：565-572. |
| 40 | LI H， LI Z L， LI X Y，et al.Selection of reference genes for real-time fluorescence quantitative PCR in different tissues and stigma development from ornamental Kale［J］.Bulletin of Botanical Research，2016，36（4）：565-572. |
| 41 | QIN H T， LI H， ABHINANDAN K，et al.Fatty acid biosynthesis pathways are downregulated during stigma development and are critical during self-Incompatible responses in ornamental kale［J］.International Journal of Molecular Sciences，2022，23（21）：13102. |
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