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Effects of Pectinase on the Distribution of Arabinogalactan Proteins in Developing Fruit of Ziziphus jujuba ‘Lingwu Changzao’
Jing WANG, Yingcai ZHANG, Shanshan TAO, Xue YANG
PDF(16234 KB)
PDF(16234 KB)
Effects of Pectinase on the Distribution of Arabinogalactan Proteins in Developing Fruit of Ziziphus jujuba ‘Lingwu Changzao’
), Shanshan TAO, Xue YANGTo explore the effects of pectinase on distribution of arabinogalactan proteins（AGPs） in fruits at different developmental stages，and to provide anatomical evidence for revealing the effects of pectinase on fruit ripening and softening， the fruits of Ziziphus jujuba ‘Lingwu Changzao’ at four different development stages were treated with three different concentrations pectinase， and AGPs epitopes analysis in situ at cellular level of fruits was performed using immunocytochemistry technique. The results showed that the fluorescence intensity of the antigens recognized by JIM13 and JIM8 and MAC204 antibodies was varied in different tissues during the development of fruit at each stage. When fruit tissues were treated with 0.028 U·mL-1（E1） pectinase， there were no obvious changes in pericarp tissue structure， AGPs antigen epitopes in cell wall surface and intercellular space of pericarp and interior parenchyma cells decreased. When fruit tissues were treated with 0.056 U·mL-1（E2） and 0.084 U·mL-1（E3）pectinase， disintegration degree of cell wall in fruit tissue increased， the amount of AGPs epitopes detected in cell wall of pericarp and inner parenchyma cells decreased gradually， an increase in pectinase concentration resulted in a greater effect of AGPs on the arrangement of all epitopes in fruit and a lower fluorescence signal. After treatment with pectinase concentration up to 0.084 U·mL-1（E3）， Calcofluor White staining revealed fluorescence attenuation or reduction to varying degrees in the cell wall region， disturbance of AGPs distribution and absence of antigen epitopes were associated with changes of cellulose assembly in cells. The results indicated that the distribution of AGPs carbohydrates differed in fruits at different stages， which was related to changes in tissue structure. The absence of AGPs glycan chains in the presence of pectinase led to the block of the establishment of correlations between cell wall components and cell wall structure remodeling， and the changes in the whole cell wall structures were induced and fruit ripening was affected.
pectinase / Ziziphus jujuba ‘Lingwu Changzao’ / fruit / arabinogalactan proteins / immunofluorescence localization
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