Objectives The path of reforming and innovating the mode of teaching in the experimental course of veterinary imaging was studied to enhance students' professional competence. Methods The College of Veterinary Medicine at Inner Mongolia Agricultural University was used to deeply reform the experimental course of veterinary imaging through school-enterprise cooperation in terms of assessment standards, implementation methods, and educational methods to improve students' qualities and meet practical requirements, etc. Results A teaching mode currently more suitable for veterinary imaging at Inner Mongolia Agricultural University was explored. The course quality of veterinary imaging was qualitatively improved after using the new teaching mode, and students' satisfaction and mastery of the course was significantly improved. Conclusions The implementation of the new mode provided a good educational environment for the training of imaging talents in the veterinary industry. It will provide a reference path for the teaching of skill-based disciplines in the future.