Objectives The path of training innovative and entrepreneurial talents in agricultural majors under the background of rural revitalization was studied. Methods Meizhou Vocational and Technical College in Guangdong Province was used to study the path of training innovative and entrepreneurial talents, with the focus on enhancing the innovation and entrepreneurship abilities of agricultural majors in vocational colleges under the background of rural revitalization. Results The path of training innovative and entrepreneurial talents in agricultural majors includes integrating innovation and entrepreneurship education into major courses, adopting an approach towards full process education, and strengthening popularization of policy and competition. The characteristic courses were set up with value education in the course. Teaching methods were expanded and "dual teacher" classrooms were built by combining local industries with characteristics. The synergistic education of industry, academia, and research was promoted to integrate resources from all three parties, and assist in rural revitalization. Teaching was improved through competitions. Various competitions were used to train the professional skills and innovative and entrepreneurial abilities of students. The incubation of achievements in researches was strengthened. Students were guided to participate in scientific researches. A technology-based incubation system was constructed to promote the transformation of achievements. Conclusions The model of training talent established is an effective path to enhance the innovative and entrepreneurial abilities of students in agricultural colleges.