Objectives The physical and chemical properties of total flavonol solution in roots, stems and leaves of hybrid Broussonetia papyrifera was studied. The differences of total flavonoids in different parts were analyzed. Methods The total flavonol solution extracted from the roots, stems and leaves of hybrid Broussonetia papyrifera Zhongke No.1-101 was used to determine the apparent characteristics, pH, electrical conductivity and antioxidant capacity with specific instruments and equipment, and then to observe the microscopic characteristics with electron scanning microscope. Results The total flavonol solution with equal volume of roots, stems, and leaves in hybrid Broussonetia papyrifera varied in color and carried a volatile taste of ethyl/methanol. The pH was between 6.3 and 7.0, with stems>roots>leaves, but the difference was not significant. There was no significant difference in electrical conductivity, all ranging from 966 μS/cm to 1 000 μS/cm. The overall scavenging rate of DPPH and ABTS free radicals under the same condition concentration was as follows: stem≥root>leaf, and root>stem≥leaf. There were significant differences in the morphology of solid after special drying treatment at the same concentration. Conclusions The total flavonol solution in the roots, stems, and leaves of hybrid Broussonetia papyrifera had no significant difference in color, pH, and electrical conductivity, but there were significant differences in the scavenging rate of free radicals and the morphology of solid after drying. The content of total flavonoid in the roots and stems of hybrid Broussonetia papyrifera was relatively high, with the potential to be developed into antioxidant additives for feed.