Objectives The construction and implementation of the textbook with new form for Analysis and Application of Marine Biology with characteristic of marine adapted to 'Internet + vocational education' was studied. Methods The content of textbook for Analysis and Application of Marine Biology was diversely developed, reconstructed, implemented and revised based on the reform of teachers, textbooks and teaching methods and relying on the core course Marine Biology Basis of high-level aquaculture technology specialty (group) in Shandong Province. Results The textbook with new form for Analysis and Application of Marine Biology with characteristic of marine adapted to 'Internet + vocational education' was constructed. An ecological chain of student-centered textbook, course, classroom, teacher and student was established. The evaluation of teaching was effectively implemented. The textbooks were periodically revised and continuously updated. Conclusions The construction and implementation of textbook with new form has effectively promoted the reform of " Teachers, Textbooks, and Teaching Methods " and provided strong support for training high skilled talents who know and love aquaculture. It will provide reference for the development and implementation of textbooks with new form for other related courses.