Objectives The effects of applying directed microecology (DME) technology in the fermentation of feed and silage were studied to solve the problems of the high cost and short shelf life faced by strains used in fermented feed. Methods Fermentation of commercially available lactic acid bacteria was expanded and verified on DME incubator. The cultured active bacterial solution was applied and evaluated in the fermentation of feed and silage. Results The key strains of lactic acid bacteria used in fermented feed were effectively expanded on the DME incubator with Lactobacillus plantarum at 30×10⁸ CFU/mL, Lactobacillus delbrueckii at 40×10⁸ CFU/mL, Lactobacillus reuteri at 20×10⁸ CFU/mL, Lactobacillus casei at 20×10⁸ CFU/mL, Pediococcus acidilactici at 50×10⁸ CFU/mL, Lactobacillus paracasei at 30×10⁸ CFU/mL, Lactobacillus acidophilus at 30×10⁸ CFU/mL, Streptococcus bovis at 10×10⁸ CFU/mL, and Enterococcus faecium at 50×10⁸ CFU/mL. Adding active lactic acid bacteria solution during the fermentation process of fermented feed, corn silage, and Caragana korshinskii silage not only improved the sensory characteristics of fermented feed, but also effectively inhibited the growth of miscellaneous bacteria in the feed. The quality of corn silage was upgraded to the first-class standard, with a comprehensive score of more than 88 points. Conclusions Directed microecology (DME) technology provides an efficient and environmentally friendly solution for the fermentation of feed and silage, which helps promote the green and sustainable development of animal husbandry and the development of microecology in feed.