Objectives The article aims to cultivate "know agriculture, love rural areas, love farmers, able to start a business, dare to start a business" talents for the agricultural, rural and farmer-related industries. Methods The article constructs a "four-in-one" specialized and entrepreneurial integration teaching model that integrates course and ideological education, job-based learning, competitions, and certifications, as well as production and education integration and practical and entrepreneurial practice integration for the animal husbandry and veterinary medicine major. The model has been repeatedly practiced. Results A number of landmark achievements have been achieved, promoting the three-teaching reform, and providing reference for cultivating the "entrepreneurial ability" of students in the animal husbandry and veterinary medicine major of vocational colleges. Conclusion The promotion of this model is the realistic demand for deepening the innovation and entrepreneurship education in colleges and universities, an important approach to improving the quality of talent cultivation in vocational colleges' animal husbandry and veterinary medicine majors, and an inevitable path for cultivating high-quality new-type agricultural and animal husbandry talents in the new era.