Objectives The effects of different proportions of WDMDG (wet milling pretreated and dehulled-moutai distiller^,s grains) substitution for soybean meal on the growth performance and serum biochemical index and immune index of Jiujin yellow chickens were studied to provide a scientific basis for WDMDG to replace soybean meal. Methods A total of 450 healthy 9-pound yellow male chickens aged one-day-old were selected and randomly divided into 3 groups, with 3 replicates in each group and 50 chickens in each replicate. The chickens in the control group were fed with a basic diet. The chickens in experiment group Ⅰand experiment group Ⅱ were fed with the basal diet enriched with 5% WDMDG, 8% WDMDG and other nutrients to replace soybean meal. The experiment lasted for 26 days. The average daily weight gain, average daily food intake, feed to weight ratio, and death and culling rate of Jiujin yellow chickens were determined. The content of TP, ALB, BUN, the activity of alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and the level of IgG, IgA, lgM in the serum of Jiujin yellow chickens were measured. Results Compared with the control group, there was no significant difference in the growth performance of Jiujin yellow chickens between experiment group I and experiment group Ⅱ (P＞0.05). The content of BUN in the serum of Jiujin yellow chickens in experimental group Ⅰ and experimental group Ⅱ increased by 4.88% (P>0.05) and 19.51% (P<0.05), respectively. There were no significant differences in the content of TP, ALB, the activity of ALT and AST, and the level of IgG, IgA, IgM in the serum of Jiujin yellow chickens among all groups (P＞0.05). Conclusions The use of 5% WDMDG and other nutrients to replace some soybean meal in the basic diet of Jiujin yellow chickens had no a significantly negative effects on the daily weight gain, feed to weight ratio, death and culling rate, serum biochemical index, and immune index of Jiujin yellow chickens.