Objectives The effects of probiotics on commercially fattening sows fed with a diet contaminated with zearalenone were studied to clarify the alleviating effect of probiotics on the toxicity of zearalenone. Methods 18 fattening sows with a body weight of (36.17 ± 2.24) kg were selected and randomly divided into 3 treatments, with 6 replicates for each treatment. The experimental period was 24 days. The three treatments for sows included control group A fed with normal diet, treatment group B added 300 µg/kg of ZEA to the feed of group A, and treatment group C added 0.2% probiotics(Bacillus licheniformis and keratase) to the feed of group B. 3 sows after ending the feeding experiment were selected from Group A, Group B, and Group C for slaughter experiments based on indexes including the redness and swelling of the vagina and growth performance. Results The diet containing 300.00 μg/kg of ZEA led to the swelling of the vagina and an increase in the index of reproductive organ in commercially fattening sows. The vaginal area of three groups of sows increased with prolonged feeding time. The redness and swelling of the vaginal area in Group C returned to the normal level after it increased. The performance of three groups of sows in terms of production performance including the daily gain of weight, feed intake, and the conversion rate of feed was roughly the same. No residue of ZEA was detected in the liver. There was no significant difference in FSH, LH, and E2 among the groups in the determination of serum indexes, while the PRL in the serum of Group B was significantly different from other groups. Conclusions Probiotics that can efficiently degrade ZEA in vitro were validated through feeding experiments to alleviate the redness and swelling of vagina in sows and reduce the harm of ZEA to animals.