Objectives The effects of EcoteChangjia powder on growth performance and intestinal tissue structure of juvenile turbot were evaluated to provide data support for the application of EcoteChangjia powder in the aquaculture of turbot and other fish species. Methods 3 000 juvenile turbots with an initial body mass of (10 ± 1) g and (40 ± 5) g were divided into two groups including the experimental group and the control group, with 3 replicates in each group. The feed in the experimental group was supplemented with 0.1% EcoteChangjia powder, while that in the control group was not treated, lasted for 21 weeks. The length of intestinal villus, myographic thickness, specific growth rate, rate of weight gain, and the fatness of juvenile turbot were measured. Results Compared with the control group, the length of intestinal villus, muscle layer thickness, and the ratio of villus length to myographic thickness in the experimental group were significantly increased by 65.8%, 44.7% and 38.9%, respectively. There was no significant difference in the rate of weight gain, specific growth rate, and the fatness. Conclusions Adding 0.1% EcoteChangjia powder to turbot feed can increase the length of intestinal villi and myographic thickness of turbot, and improve its intestinal tissue structure.