目的 掌握贵州省玉屏县某规模养殖场猪乙型脑炎的免疫情况，为规模养殖场乙型脑炎防控提供参考。 方法 2020—2022年随机采集已筛除乙型脑炎野毒感染再接种乙型脑炎疫苗猪群的3 960份猪血清样品，采用ELISA抗体检测试剂盒进行乙型脑炎抗体检测。 结果 该场2020—2022年乙型脑炎平均免疫合格率分别为97.20%、98.33%、98.56%，总体免疫合格率为98.03%。第一季度抗体水平较低，平均免疫合格率为96.36%，三季度抗体水平最高，为99.60%；公猪和哺乳母猪抗体水平较高，免疫合格率分别为98.89%、98.26%，妊娠母猪最低，为97.73%。 结论 该猪场乙型脑炎免疫水平整体较好，但存在一定的时间和畜群差异，需加强饲养管理和免疫监测等工作。

Objectives The immunization status of Japanese encephalitis in pigs from a large-scale farm in Yuping County, Guizhou Province was investigated to provide reference for the prevention and control of Japanese encephalitis in large-scale farms. Methods 3 960 serum samples were randomly collected from pig herds that had been screened for wild-type Japanese encephalitis infection and vaccinated with the Japanese encephalitis vaccine from 2020 to 2022. ELISA antibody detection kits were used to detect Japanese encephalitis antibody. Results The average immunization qualification rate for Japanese encephalitis at the farm from 2020 to 2022 was 97.20%, 98.33%, and 98.56%, respectively, with an overall immunization qualification rate of 98.03%. The level of antibody was relatively low in the first quarter, with an average immunization qualification rate of 96.36%. The level of antibody was the highest in the third quarter, at 99.60%. The level of antibody in boars and lactating sows was relatively high, with immunization qualification rate of 98.89% and 98.26%, respectively. Pregnant sows had the lowest immunization qualification rate, at 97.73%. Conclusions The overall immune level of Japanese encephalitis in this pig farm was good, but there were certain differences in time and herd. It is necessary to strengthen the management of feeding and the monitoring of immunization.