目的 探讨定向微生态（DME）技术在饲料发酵和青贮发酵中的应用效果，旨在解决发酵饲料应用菌株面临的成本高、货架期短等问题。 方法 对市售乳酸菌在DME培菌机设备上进行发酵验证，并将培养获得的活性菌液在饲料和青贮发酵上进行应用评估。 结果 发酵饲料关键应用乳酸菌菌株在DME培菌机上可以实现植物物乳杆菌30×10⁸ CFU/mL、德式乳杆菌40×10⁸ CFU/mL、罗伊氏乳杆菌20×10⁸ CFU/mL、干酪乳杆菌20×10⁸ CFU/mL、乳酸片球菌50×10⁸ CFU/mL、副干酪乳杆菌30×10⁸ CFU/mL、嗜酸乳杆菌30×10⁸ CFU/mL、戊糖片球菌10×10⁸ CFU/mL、粪肠球菌50×10⁸ CFU/mL的有效扩培，且在发酵饲料、玉米青贮和柠条青贮的发酵过程中添加活性乳酸菌菌液不仅能改善发酵饲料的感官特性，还能有效抑制饲料中杂菌生长，玉米青贮料的品质提升至一级标准，综合评分可达88分以上。 结论 定向微生态技术为饲料及青贮发酵提供了一种高效、环保的解决方案，有助于推动畜牧业的绿色可持续发展和饲用微生态的发展。

Objectives The effects of applying directed microecology (DME) technology in the fermentation of feed and silage were studied to solve the problems of the high cost and short shelf life faced by strains used in fermented feed. Methods Fermentation of commercially available lactic acid bacteria was expanded and verified on DME incubator. The cultured active bacterial solution was applied and evaluated in the fermentation of feed and silage. Results The key strains of lactic acid bacteria used in fermented feed were effectively expanded on the DME incubator with Lactobacillus plantarum at 30×10⁸ CFU/mL, Lactobacillus delbrueckii at 40×10⁸ CFU/mL, Lactobacillus reuteri at 20×10⁸ CFU/mL, Lactobacillus casei at 20×10⁸ CFU/mL, Pediococcus acidilactici at 50×10⁸ CFU/mL, Lactobacillus paracasei at 30×10⁸ CFU/mL, Lactobacillus acidophilus at 30×10⁸ CFU/mL, Streptococcus bovis at 10×10⁸ CFU/mL, and Enterococcus faecium at 50×10⁸ CFU/mL. Adding active lactic acid bacteria solution during the fermentation process of fermented feed, corn silage, and Caragana korshinskii silage not only improved the sensory characteristics of fermented feed, but also effectively inhibited the growth of miscellaneous bacteria in the feed. The quality of corn silage was upgraded to the first-class standard, with a comprehensive score of more than 88 points. Conclusions Directed microecology (DME) technology provides an efficient and environmentally friendly solution for the fermentation of feed and silage, which helps promote the green and sustainable development of animal husbandry and the development of microecology in feed.