目的 探讨芒果苷（MGF）通过调控Wnt/β-连环蛋白（β-catenin）信号通路对大鼠髋部骨折愈合的作用，阐明其相关作用机制。 方法 建立SD大鼠髋部骨折模型，造模成功后将大鼠分为模型组、MGF组和MGF+XAV-939（Wnt/β-catenin信号通路抑制剂）组，另设假手术组，每组15只。术后第1天各组大鼠按分组方法给予MGF或XAV-939干预，每2 d给药1次，共14次。采用Lane-Sandhu X射线评分法评估术后第2和4周各组大鼠骨折愈合情况，显微CT（Micro CT）检测各组大鼠骨显微结构参数骨体积（BV）、骨小梁数量（Tb.N）、骨体积分数（BV/TV）和骨小梁厚度（Tb.Th），HE染色观察各组大鼠骨痂组织形态表现，酶联免疫吸附试验（ELISA）法检测各组大鼠血清中成骨标志物骨碱性磷酸酶（BALP）和Ⅰ型前胶原N端前肽（PINP）及破骨标志物抗酒石酸酸性磷酸酶5b（TRACP-5b）和Ⅰ型胶原羧基末端肽（CTX）水平，Western blotting法检测各组大鼠骨痂组织中β-catenin、Runt相关转录因子2（Runx2）和骨形态发生蛋白2（BMP-2）蛋白表达水平。 结果 与假手术组比较，模型组大鼠骨折后第2和4周骨折线清晰，骨折部位存在较多纤维组织，未发现骨性骨痂，Lane-Sandhu X射线评分及骨显微结构参数BV、Tb.N、BV/TV和Tb.Th均降低（P<0.05），血清中BALP水平降低（P<0.05），PINP、TRACP-5b和CTX水平升高（P<0.05），骨痂组织中β-catenin、Runx2和BMP-2蛋白表达水平降低（P<0.05）。与模型组比较，MGF组大鼠骨折后第2周新生骨痂明显增多，第4周骨折线基本消失，骨折愈合较快，骨折部位大量纤维组织被骨组织替代，骨性骨痂量明显增多，Lane-Sandhu X射线评分及骨显微结构参数BV、Tb.N、BV/TV和Tb.Th均升高（P<0.05），血清中BALP水平升高（P<0.05），PINP、TRACP-5b和CTX水平降低（P<0.05），骨痂组织中 β-catenin、 Runx2 和 BMP-2 蛋白表达水平升高（P<0.05）。与 MGF组比较，MGF+XAV-939组大鼠骨折后第2和4周骨折部位仅有少量新生骨痂，髓腔未形成，Lane-Sandhu X射线评分及骨显微结构参数BV、Tb.N、BV/TV和Tb.Th均降低（P<0.05），血清中BALP水平降低（P<0.05），PINP、TRACP-5b和CTX水平升高（P<0.05），骨痂组织中β-catenin、Runx2和BMP-2蛋白表达水平降低（P<0.05）。 结论 MGF可促进大鼠髋部骨折愈合，其作用机制可能与激活Wnt/β-catenin信号通路有关。

Objective To discuss the effect of mangiferin （MGF） on the healing of hip fracture in the rats by regulating the Wnt/β-catenin signaling pathway， and to clarify the mechanism. Methods The hip fracture model in the SD rats was established. After successful modeling， the rats were divided into model group， MGF group， and MGF+XAV-939 （Wnt/β-catenin signaling pathway inhibitor） group， and sham operation group was set up， and there were 15 rats in each group. From the first day after surgery， the rats in each group received MGF or XAV-939 interventions every two days， totally for 14 times. Lane-Sandhu X-ray scoring method was used to detect the fracture healing in the second and fourth weeks after surgery； micro CT was used to detect the microstructural parameters of the bone， such as bone volume （BV）， number of bone trabeculae （Tb.N）， bone volume fraction （BV/TV）， and bone trabecular thickness （Tb.Th）； HE staining was used to observe the morphology of callus tissue of the rats in various groups； enzyme-linked immunosorbent assay （ELISA） was used to detect the levels of osteogenic markers bone alkaline phosphatase （BALP） and type Ⅰ procollagen N-terminal propeptide （PINP） and bone resorption markers tartrate resistant acid phosphatase-5b （TRACP-5b） and Carboxy-terminal terminalpeptide of type Ⅰ collagen （CTX） in serum of the rats in various groups；Western blotting method was used to detect the expression levels of β-catenin， Runt-associated transcription factor 2 （Runx2）， and bone morphogenetic protein-2 （BMP-2） in callus tissue of the rats in various groups. Results Compared with sham operation group， the rats in model group showed distinct fracture lines， an abundance of fibrous tissue at the fracture site， and no bony callus at the 2nd and 4th weeks after fracture， the Lane-Sandhu X-ray scores and microstructural parameters BV， Tb.N， BV/TV， and Tb.Th of the rats in model group were decreased （P<0.05），the serum level of BALP was decreased （P<0.05）， while the levels of PINP， TRACP-5b， and CTX were increased （P<0.05），and the expression levels of β-catenin， Runx2， and BMP-2 proteins in callus tissue were decreased （P<0.05）. Compared with model group， the rats in MGF group showed significantly increased new bone callus at 2nd weeks after fracture， almost no visible fracture line at the 4th weeks， faster fracture healing， and replacement of fibrous tissue by bone tissue at the fracture site， the Lane-Sandhu X-ray scores and microstructural parameters BV， Tb.N， BV/TV， and Tb.Th of the rats in MGF group were increased （P<0.05）， the serum level of BALP was increased （P<0.05）， the levels of PINP， TRACP-5b， and CTX were decreased （P<0.05），and the expression levels of β-catenin， Runx2， and BMP-2 proteins in callus tissue were increased （P<0.05）. Compared with MGF group， the rats in MGF+XAV-939 group showed only a small amount of new bone callus at the fracture site at 2nd and 4th weeks after fracture， with no formation of the marrow cavity， and the Lane-Sandhu X-ray scores and microstructural parameters BV， Tb.N， BV/TV， and Tb.Th of the rats in MGF+XAV-939 group were decreased （P<0.05）， the serum level of BALP was decreased （P<0.05）， the levels of PINP， TRACP-5b， and CTX were increased （P<0.05），and the expression levels of β-catenin， Runx2， and BMP-2 proteins in callus tissue were decreased （P<0.05）. Conclusion MGF can promote the healing of hip fracture in the rats， and its mechanism may be related to activation of Wnt/β-catenin signaling pathway.