目的 探讨微小RNA-151（miR-151）对低氧条件下人绒毛膜滋养层细胞HTR-8/SVneo生物学行为的影响，并阐明其可能的作用机制。 方法 选择47例子痫前期产妇（子痫前期组）和36例正常产妇（正常组）作为研究对象，采用实时荧光定量PCR（RT-qPCR）法检测２组产妇胎盘组织中miR-151表达水平。将miR-151 inhibitor及其阴性对照inhibitor NC转染至HTR-8/SVneo细胞中，进行低氧（1% O₂）干预48 h，设立对照组、低氧组、低氧+inhibitor NC组和低氧+inhibitor组。采用RT-qPCR法检测各组细胞中miR-151表达水平，MTT法检测各组细胞存活率，Transwell小室实验检测各组迁移细胞数和侵袭细胞数，Western blotting法检测各组细胞中基质金属蛋白酶（MMP）-2和MMP-9及上皮间质转化（EMT）相关蛋白表达水平。采用生物信息学分析预测miR-151下游靶基因，并结合STRING数据库对交集靶基因进行蛋白-蛋白互作（PPI）网络分析。 结果 与正常组比较，子痫前期组产妇胚胎组织中miR-151表达水平明显升高（P<0.05）。与对照组比较，低氧组HTR-8/SVneo细胞存活率、迁移细胞数和侵袭细胞数及细胞中MMP-2、MMP-9、N-钙黏蛋白和波形蛋白表达水平明显降低（P<0.05），miR-151和E-钙黏蛋白表达水平明显升高（P<0.05）。与低氧组比较，低氧+inhibitor组HTR-8/SVneo细胞中MMP-2、MMP-9、N-钙黏蛋白和波形蛋白表达水平明显升高（P<0.05），miR-151和E-钙黏蛋白水平明显降低（P<0.05），而低氧+inhibitor NC组上述指标差异无统计学意义（P>0.05）。生物信息学分析，miR-151下游有34个潜在靶基因，其中环指CCCH-型锌指蛋白域蛋白1（RC3H1）、AGO2、AGO3、脆性X相关蛋白1（FXR1）和转化因子2β（TRA2B）可能是关键潜在靶基因。 结论 miR-151在子痫前期患者胎盘组织中高表达，下调miR-151表达可促进低氧条件下滋养层细胞的增殖、迁移和侵袭。

Objective To discuss the effect of microRNA-151 （miR-151） on the biological behavior of the human trophoblast cells HTR-8/SVneo under hypoxic conditions，and to clarify the potential mechanism. Methods A total of 47 parturients with preeclampsia （preeclampsia group） and 36 parturients （ normal group） were selected as the research subjects. The expression level of miR-151 in placenta tissue of the subjects in two groups was detected by real-time fluorescence quantitative PCR （RT-qPCR） method. The miR-151 inhibitor and its negative control inhibitor NC were transfected into the HTR-8/SVneo cells， followed by exposure to hypoxia （1% O₂） for 48 h to establish control， hypoxia， hypoxia + inhibitor NC， and hypoxia + inhibitor groups. RT-qPCR method was used to detect the expression levels of miR-151 in the cells in various groups； MTT assay was used to detect the survival rates of the cells in various groups； Transwell chamber assay was used to detect the migration and invasion numbers of the cells in various groups； Western blotting method was used to detect the expression levels of matrix metalloproteinases （MMP）-2 and MMP-9， and epithelial-mesenchymal transition （EMT） related proteins in the cells in various groups； Bioinformatics analysis was used to predict the downstream target genes of miR-151， and the intersection target genes were further analyzed for protein-protein interaction （PPI） network by STRING Database. Results Compared with normal group， the expression level of miR-151 in placenta tissue of the patients in preeclampsia group was significantly increased （P<0.05）. Compared with control group， the proliferation activity， number of invasion cells， and number of migration cells of HTR-8/SVneo cells in hypoxia group were significantly decreased （P<0.05）， the expression levels of MMP-2， MMP-9， N-cadherin， and vimentin in the cells were significantly decreased （P<0.05）， and the expression levels of miR-151 and E-cadherin were significantly increased （P<0.05）. Compared with hypoxia group， the expression levels of MMP-2， MMP-9， N-cadherin， and vimentin in the cells in hypoxia + inhibitor group were significantly increased （P<0.05）； the levels of miR-151 and E-cadherin were significantly decreased （P<0.05）； while there were no significant differences in the above indexes in hypoxia + inhibitor NC group （P>0.05）. The bioinformatics analysis results showed that 34 potential target genes of miR-151， among which RCCCH-type zinc finger protein 1 （RC3H1）， AGO2， AGO3， Fragile X related protein 1 （FXR1）， and transformer 2β （TRA2B） may be the key potential target genes. Conclusion miR-151 is highly expressed in placenta tissue of the patients with preeclampsia. The downregulation of miR-151 expression can promote the proliferation， invasion， and migration of the trophoblast cells under hypoxic conditions.