目的 基于网络药理学探讨王浆酸（10-HDA）对人结肠癌SW620细胞增殖和迁移的影响，阐明其相关分子机制。 方法 利用中药系统药理学（TMSCP）数据库和中医药综合数据库（TCMID）以关键词“蜂王浆”进行检索得到10-HDA等活性成分及对应靶点，采用Swiss Target Prediction数据库预测小分子靶点。采用GeneCards数据库和在线人类孟德尔遗传（OMIM）数据库以关键词“Colon Cancer”获得靶点，利用String数据库和Cytoscape 3.8.0软件构建蛋白-蛋白互作（PPI）网络，筛选核心靶点；利用Metascape数据库对基因本体（GO）功能富集和京都基因与基因组百科全书（KEGG）信号通路进行富集分析，筛选特有成分10-HDA进行体外活性实验。将生长状态良好的人结肠癌SW620细胞分为对照组和不同剂量（1、5、10、15和20 mmol·L^－1）10-HDA组，采用MTT法检测各组细胞活性并计算细胞存活率。SW620细胞分为对照组、低剂量（5 mmol·L^－1） 10-HDA组、中剂量 （10 mmol·L^－1） 10-HDA组和高剂量（15 mmol·L^－1） 10-HDA组， Hoechst33342染色法观察各组细胞形态表现，细胞划痕实验检测各组细胞划痕愈合率，流式细胞术检测各组不同细胞周期细胞百分率，生化法检测各组细胞中总抗氧化能力（T-AOC）和超氧化物歧化酶（SOD）活性，Western blotting法检测各组细胞中B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、含半胱氨酸的天冬氨酸蛋白水解酶3（Caspase-3）、含半胱氨酸的天冬氨酸蛋白水解酶9（Caspase-9）、糖原合成酶激酶3β（GSK3β）、β-连环蛋白（β-catenin）和细胞周期蛋白D1（CyclinD1）蛋白表达水平。 结果 TCMSP数据库筛选得到蜂王浆6种活性成分，10-HDA治疗结肠癌核心靶点28个。GO功能富集分析主要涉及细胞增殖和细胞凋亡等信号通路；KEGG信号通路富集分析涉及细胞周期、前列腺癌、细胞衰老和p53等信号通路，GSK3β/β-catenin信号通路与细胞周期有密切关联。与对照组比较，5、10、15和20 mmol·L^－1 10-HDA组细胞存活率呈剂量依赖性降低（P<0.05或P<0.01）。与对照组比较，不同剂量10-HDA组细胞中凋亡细胞数明显增多，细胞划痕愈合率明显降低（P<0.05或P<0.01），中和高剂量10-HDA组细胞中S期细胞百分率明显升高（P<0.05或P<0.01），不同剂量10-HDA组细胞中T-AOC和SOD活性明显降低（P<0.05或P<0.01）。与对照组比较，低剂量10-HDA组细胞中Bcl-2蛋白表达水平明显降低（P<0.01），GSK3β蛋白表达水平明显升高（P<0.05）；与对照组比较，中和高剂量10-HDA组细胞中Bax、Caspase-3、Caspase-9和GSK3β蛋白表达水平明显升高（P<0.05或P<0.01），Bcl-2、β-catenin和CyclinD1蛋白表达水平明显降低（P<0.01）。 结论 10-HDA可明显抑制结肠癌细胞增殖和迁移，并可促进结肠癌细胞的凋亡和氧化水平，其作用机制可能与激活GSK3β/β-catenin信号通路有关。

Objective To discuss the effect of royal jelly acid （10-HDA） on the proliferation and migration of the human colon cancer SW620 cells based on the network pharmacology， and to clarify its related molecular mechanism. Methods The active ingredients such as 10-HDA and their corresponding targets were retrieved by using the keyword “royal jelly” from the Traditiomal Chinese Medicine Systems Pharmacology （TMSCP）Database and the Traditiomal Chinese Medicine Integrated Database （TCMID）； the small molecule targets were predicted by the Swiss Target Prediction Database.The GeneCards Database and the Online Mendelian Inheritance in Man（OMIM） Database were used to obtain the targets with the keyword “Colon Cancer”；the protein-protein interaction （PPI） network was constructed by using the String Database and Cytoscape 3.8.0 Software to screen the core targets； the Gene Ontology （GO） function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes （KEGG） signaling pathway enrichment analysis were analyzed by Metascape Database； the specific ingredient 10-HDA was screened for the in vitro activity experiments. The human colon cancer SW620 cells with good growth status were divided into control group and different doses （1， 5， 10， 15， and 20 mmol·L^－1） of 10-HDA groups. The viabilities of the cells in various groups were detected by MTT method and the survival rates of the cells were calculated. The SW620 cells were divided into control group， low dose （5 mmol·L^－1） of 10-HDA group， middle dose （10 mmol·L^－1） of 10-HDA group， and high dose （15 mmol·L^－1） of 10-HDA group； Hoechst33342 staining method was used to observe the morphology of the cells in various groups； cell scratch test was used to detect the scratch healing rates of the cells in various groups；flow cytometry was used to detect the percentages of the cells at different cell cycles in various groups； biochemical method was used to detect the activities of total antioxidant capacity （T-AOC） and superoxide dismutase （SOD） in the cells in various groups； Western blotting method was used to detect the expression levels of B-cell lymphoma 2 （Bcl-2）， Bcl-2-associated X protein （Bax），cysteine-containing aspartate proteolytic enzyme-3 （Caspase-3）， cysteine-containing aspartate proteolytic enzyme-9 （Caspase-9）， glycogen synthase kinase 3β （GSK3β）， β-catenin， and cyclin D1 proteins in the cells in various groups. Results Six active ingredients of royal jelly were screened out by the TCMSP Database， and 28 core targets of 10-HDA in the treatment of colon cancer were obtained. The GO function enrichment analysis mainly included the signaling pathways such as cell proliferation and apoptosis. The KEGG signaling pathway enrichment analysis included the cell cycle， prostate cancer， cell senescence， and p53 signaling pathways； the GSK3β/β-catenin signaling pathway was closely related to the cell cycle. Compared with control group， the viabilities of the cells in 5，10，15， and 20 mmol·L^－110-HDA groups were decreased in a dose-dependent manner （P<0.05 or P<0.01）， the numbers of apoptotic cells in different doses of 10-HDA groups were significantly increased， and the scratch healing rates of the cells were significantly decreased （P<0.05 or P<0.01）； the percentages of the cells at S phase in middle and high doses of 10-HDA groups were significantly increased （P<0.05 or P<0.01），the activities of T-AOC and SOD in the cells in different doses of 10-HDA groups were significantly decreased （P<0.05 or P<0.01）. Compared with control group， the expression level of Bcl-2 protein in the cells in low dose of 10-HDA group was significantly decreased （P<0.01）， and the expression level of GSK3β protein was significantly increased （P<0.05）； compared with control group， the expression levels of Bax， Caspase-3， Caspase-9， and GSK3β proteins in the cells in middle and high doses of 10-HDA groups were significantly increased （P<0.05 or P<0.01）， and the expression levels of Bcl-2， β-catenin，and CyclinD1 proteins were significantly decreased （P<0.01）. Conclusion 10-HDA can significantly inhibit the proliferation and migration of the colon cancer cells and promote the apoptosis and oxidation levels of the colon cancer cells，and its mechanism may be related to the activation of the GSK3β / β-catenin signaling pathway.