目的 探讨CD40配体（CD40L）通过长链非编码RNA（lncRNA） linc00239对人单核细胞白血病THP-1细胞生物学行为的影响，阐明其可能的作用机制。 方法 构建linc00239过表达载体（pcDNA-linc00239）和干扰载体（sh-linc00239），转染至THP-1细胞，采用实时荧光定量PCR（RT-qPCR）法检测转染效率。THP-1细胞分为对照组、空载体（vector）组、pcDNA-linc00239组、sh-linc00239组、vector+CD40L组、pcDNA-linc00239+CD40L组和sh-linc00239+CD40L组。RT-qPCR法检测各组细胞中linc00239表达水平，CCK-8法检测各组细胞增殖活性，流式细胞术检测各组不同细胞周期细胞百分率和细胞凋亡率，RT-qPCR法和Western blotting法检测各组细胞中B细胞淋巴瘤2（Bcl-2）和Bcl-2关联X蛋白（Bax）mRNA和蛋白表达水平，Western blotting法检测各组细胞中蛋白激酶B（AKT）和磷酸化AKT（p-AKT）蛋白表达水平并计算p-AKT/AKT比值。 结果 与vector组比较，pcDNA-linc00239组细胞增殖活性和G₂期细胞百分率明显升高（P<0.05或P<0.01），细胞中linc00239、Bcl-2 mRNA及蛋白表达水平和p-AKT/AKT比值明显升高（P<0.05或P<0.01），G₁期细胞百分率、细胞凋亡率和细胞中Bax mRNA及蛋白表达水平明显降低（P<0.05）；与vector组比较，sh-linc00239组和vector+CD40L组细胞增殖活性和G₂期细胞百分率明显降低（P<0.05或P<0.01），细胞中linc00239、Bcl-2 mRNA和蛋白表达水平及p-AKT/AKT比值明显降低（P<0.05或P<0.01），G₁期细胞百分率、细胞凋亡率和细胞中Bax mRNA及蛋白表达水平明显升高（P<0.05或P<0.01）。与pcDNA-linc00239组比较，pcDNA-linc00239+CD40L组细胞增殖活性和G₂期细胞百分率明显降低（P<0.05或P<0.01），细胞中linc00239、Bcl-2 mRNA和蛋白表达水平及p-AKT/AKT比值明显降低（P<0.05或P<0.01），G₁期细胞百分率、细胞凋亡率和细胞中Bax mRNA及蛋白表达水平明显升高（P<0.05或P<0.01）；与sh-linc00239组比较，sh-linc00239+CD40L组细胞增殖活性和G₂期细胞百分率明显降低（P<0.05或P<0.01），细胞中linc00239、Bcl-2 mRNA和蛋白表达水平及p-AKT/AKT比值明显降低（P<0.05或P<0.01），G₁期细胞百分率、细胞凋亡率和细胞中Bax mRNA及蛋白表达水平明显升高（P<0.05或P<0.01）。 结论 CD40L可通过linc00239抑制THP-1细胞增殖和细胞周期进展，并诱导细胞凋亡。

Objective To discuss the effect of CD40 ligand （CD40L） on the biological behavior of the human monocytic leukemia THP-1 cells through long non-coding RNA（lncRNA） linc00239，and to clarify its potential mechanism. Methods The linc00239 over-expression vector （pcDNA-linc00239） and interference vector （sh-linc00239） were constructed and transfected into the THP-1 cells.Real-time fluorescence quantitative PCR （RT-qPCR） method was used to detect the transfection efficiency. The THP-1 cells were divided into control group， vector group， pcDNA-linc00239 group， sh-linc00239 group， vector+CD40L group， pcDNA-linc00239+CD40L group， and sh-linc00239+CD40L group. RT-qPCR method was used to detect the expression levels of linc00239 in the cells in various groups； CCK-8 assay was used to detect the proliferation activities of the cells in various groups；flow cytometry was used to detect the percentages of the cells at different cell cycles and the apoptotic rates of the cells in various groups；RT-qPCR and Western blotting methods were used to to detect the expression levels of B-cell lymphoma-2 （Bcl-2） and Bcl-2-associated X protein （Bax） mRNA and proteins in the cells in various groups； Western blotting method was used to detect the expression levels of protein kinase B （AKT） and phosphorylated AKT （p-AKT） proteins in the cells in various groups，and the ratio of p-AKT/AKT was calculated. Results Compared with vector group， the proliferation activity of the cells and the percentage of the cells at G₂ phase in pcDNA-linc00239 group were significantly increased （P<0.05 or P<0.01）， the expression levels of linc00239， Bcl-2 mRNA and protein， and the ratio of p-AKT/AKT were significantly increased （P<0.05 or P<0.01），the percentage of the cells at G₁ phase， apoptotic rate， and expression levels of Bax mRNA and protein in the cells were significantly decreased （P<0.05）； compared with vector group， the proliferation activity of the cells and percentage of the cells at G₂ phase， expression levels of linc00239， Bcl-2 mRNA and protein， and ratio of p-AKT/AKT in the cells in sh-linc00239 group and vector+CD40L group were significantly decreased （P<0.05 or P<0.01）， while the percentage of the cells at G₁ phase， apoptotic rate， and the expression levels of Bax mRNA and protein in the cells were significantly increased （P<0.05 or P<0.01）；compared with pcDNA-linc00239 group， the proliferation activity of the cells and percentage of cells at G₂ phase in pcDNA-linc00239+CD40L group were significantly decreased （P<0.05 or P<0.01）， the expression levels of linc00239， Bcl-2 mRNA and protein，and ratio of p-AKT/AKT were significantly decreased （P<0.05 or P<0.01），while the percentage of cells at G₁ phase， apoptotic rate， and the expression levels of Bax mRNA and protein were significantly increased （P<0.05 or P<0.01）；compared with sh-linc00239 group， the proliferation activity of the cells and percentage of cells at G₂ phase in sh-linc00239+CD40L group were significantly decreased （P<0.05 or P<0.01）， the expression levels of linc00239， Bcl-2 mRNA and protein， and ratio of p-AKT/AKT were significantly decreased （P<0.05 or P<0.01），and the percentage of the cells at G₁ phase， apoptotic rate， and expression levels of Bax mRNA and protein were significantly increased （P<0.05 or P<0.01）. Conclusion CD40L can inhibit the proliferation and cell cycle progression of the THP-1 cells through linc00239 and induce the apoptosis.